Article Figures & Data
Figures
- Fig. 1.
Radiation hybrid analysis. The hRAD51 genomic locus, CYP19, and several additional microsatellite markers were mapped to chromosome 15q14–15 using the radiation hybrid method (see “Materials and Methods” for details). Markers used in LOH studies are underlined.
- Fig. 2.
hRad51 genomic structure. A human BAC clone containing the hRad51 gene was identified by screening a BAC library (Research Genetics). Exon-intron borders were determined by direct sequencing of the BAC DNA. A, hRad51 consists of 10 exons (dark gray box, coding exons; light gray box, noncoding exons) and covers ∼30 kb (*, introns sequenced completely). B, exon-intron borders follow the GT-AG rule. Capital letters, intronic sequences. Numbers in parentheses, size (in bp) of the published cDNA sequence (GenBank accession number D14134).
- Fig. 3.
Analysis of the CpG island in the 5′ region of the hRad51 gene. The 5′ region of the hRad51 gene was sequenced and analyzed using the GCG Software Package (GCG, Madison, WI). The translation start site (ATG) was used as 0. According to the rules established by Gardiner-Garden and Frommer (43) , a region of 989 bp can be considered a CpG island (▪), which covers exon 1 (□) and extends into intron 1. The region marked by the
was examined for changes in DNA methylation.
Tables
- Table 1
Primers used for mutational analysis
Exon 2 CAAGCCCCTTATTTCTCTAGT R37; 5′ CCTTCCACTAGGTAGAAGAA R38; 3′ Exon 3 GGACACATAACATCTGTGTTAG R39; 5′ TGTACTATTCCCACTAATGCCT R40; 3′ Exon 4 TCAAGATCACTGTGGTAAGGA R41; 5′ GCTTTCCTAACTAGAGTTCAC R42; 3′ Exon 5 CCAAGAACATTTCTATGACTACAG R43; 5′ CAGGAATGAAGTAATGCTTGC R44; 3′ Exon 6 CTTGGTCAGCTGTATCAGAAAT R45; 5′ GATAAGTGTAGCCATAGTCTCT R45; 3′ Exon 7 AGTTCTGTGTGCAGCCTAAA R47; 5′ GGGAAGGACTCTTAAGAACAT R48; 3′ Exon 8 ACAGGCTAGAAATAGGCTTCA R49; 5′ CTGAAAGTAGGCATTCTCTGT R50; 3′ Exon 9 GTCTATGGCCACAAAATTGACA R51; 5′ TCCGAAAAGAAGAACTGATCC R52; 3′ Exon 10 CAAAGTCAGGAACGGAATTGT R53; 5′ R54; 3′
Volume 59, Issue 18
