High Frequency of Chromosome 3p Deletion in Histologically Normal Nasopharyngeal Epithelia from Southern Chinese

Andrew Siu Chung Chan; Ka Fai To; Kwok Wai Lo; Ko Fung Mak; Wai Pak; Brian Chiu; Gary M. K. Tse; Min Ding; Xianghong Li; Joseph Chuen Kwun Lee; Dolly Poon Huang

DOI: Published October 2000

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Fig. 1.
Chromosome 3p deletion and presence of EBER transcripts in the neoplastic and nonneoplastic nasopharyngeal samples from high-risk and low-risk regions of NPC.
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Fig. 2.
Examples of chromosome 3p deletion and EBER-ISH in neoplastic and nonneoplastic nasopharyngeal samples from southern Chinese. A (Case 1, S-NP 1; Case 4, S-NP 4; Case 11, S-NP-11), histologically normal nasopharyngeal epithelia. B (Case 4, DNP-4; Case 7, DNP-7), dysplastic nasopharyngeal epithelia (Case 4, low-grade dysplastic lesion; Case 7, high-grade dysplastic lesion). C (case 4, S-NPC 4), undifferentiated NPC. H&E, ×200. EBER-ISH, ISH for EBER transcripts (×200). N, normal tissue; T, tumor. ^, LOH.

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Table 1

Chromosome 3p LOH and distribution of sex and age in neoplastic and nonneoplastic nasopharyngeal tissues from high-risk and low-risk regions for NPC

	Normal NP^a				NPC^c
	Case no.	Gender	Age	3p LOH^b	Case no.	Gender	Age	3p LOH^b
High-risk region
Hong Kong (southern Chinese)	S-NP1	F	27	+	S-NPC1	M	45	+
	S-NP2	M	41	−	S-NPC2	M	35	+
	S-NP3	F	11	+	S-NPC3	M	45	+
	S-NP4	F	17	+	S-NPC4	M	35	+
	S-NP5	M	15	+	S-NPC5	F	73	+
	S-NP6	F	16	+	S-NPC6	M	35	+
	S-NP7	M	19	+	S-NPC7	M	70	+
	S-NP8	F	26	−	S-NPC8	M	43	+
	S-NP9	M	44	+	S-NPC9	M	31	+
	S-NP10	M	38	+	S-NPC10	M	50	+
	S-NP11	F	33	+	S-NPC11	M	41	+
	S-NP12	M	39	−	S-NPC12	M	65	+
	S-NP13	M	55	+	S-NPC13	M	36	+
	S-NP14	F	79	−	S-NPC14	F	71	+
	S-NP15	M	79	+	S-NPC15	M	43	+
	S-NP16	M	68	−	S-NPC16	M	66	+
	S-NP17	F	42	+	S-NPC17	F	51	+
	S-NP18	M	94	+	S-NPC18	M	69	+
	S-NP19	M	85	+	S-NPC19	M	52	+
	S-NP20	M	46	+	S-NPC20	M	69	+
	S-NP21	M	69	+	S-NPC21	M	44	+
	S-NP22	M	41	−
	S-NP23	F	72	+
		M:F, 14:9	Mean, 45.9	17/23 (73.9%)		M:F, 18:3	Mean, 50.9	21/21 (100%)
Low-risk region
Anhui/Beijing	N-NP1	M	28	−	N-NPC1	M	47	+
(northern Chinese)	N-NP2	M	50	−	N-NPC2	M	67	+
	N-NP3	F	30	−	N-NPC3	M	40	+
	N-NP4	F	36	+	N-NPC4	M	53	+
	N-NP5	M	46	−	N-NPC5	M	35	+
	N-NP6	F	34	−	N-NPC6	M	39	+
	N-NP7	M	34	+	N-NPC7	F	50	+
	N-NP8	M	18	−	N-NPC8	M	25	−
	N-NP9	M	11	−	N-NPC9	M	60	+
	N-NP10	M	42	−	N-NPC10	M	58	−
	N-NP11	M	76	−	N-NPC11	M	62	+
	N-NP12	M	54	−	N-NPC12	M	56	+
	N-NP13	M	53	+
	N-NP14	M	55	−
	N-NP15	M	45	−
	N-NP16	M	54	−

Toronto Canada	C-NP1	F	76	−	C-NPC1	M	37	+
(Caucasians)	C-NP2	M	66	−	C-NPC2	M	74	+
	C-NP3	F	69	−	C-NPC3	M	63	+
	C-NP4	M	55	+	C-NPC4	M	59	+
	C-NP5	F	68	−	C-NPC5	M	48	+
	C-NP6	F	64	−	C-NPC6	M	55	+
	C-NP7	F	61	−	C-NPC7	F	66	+
	C-NP8	M	80	−	C-NPC8	F	54	−
	C-NP9	F	57	−	C-NPC9	M	35	−
	C-NP10	M	31	−
	C-NP11	M	41	+
	C-NP12	F	27	−
	C-NP13	F	67	+
	C-NP14	F	42	−
		M:F, 18:12	Mean, 49	6/30 (20%)		M:F, 18:3	Mean, 51.6	17/21 (81%)

^a High-risk region, n = 23; Low-risk region, n = 30.
^b +, LOH on at least one microsatellite marker on chromosome 3p; −, no LOH at all informative loci on chromosome 3p.
^c High-risk region, n = 21; Low-risk region, n = 21.

Table 2

The association of LOH on chromosome 3p and presence of EBER transcripts in the neoplastic and nonneoplastic nasopharyneal samples from high- and low-risk regions of NPC

	High-risk region		Low-risk regions
	Southern Chinese (Hong Kong)		Total of all regions		Central/Northern Chinese (Anhui/Beijing)		Caucasians (Toronto, Canada)
	3p LOH	EBER	3p LOH	EBER	3p LOH	EBER	3p LOH	EBER
NPC	100% (21/21)	100% (21/21)	81% (17/21)	90.5% (19/21)	83.3% (10/12)	100% (12/12)	77.8% (7/9)	77.8% (7/9)
Normal NP^a	73.9% (17/23)	0% (0/23)	20% (6/30)	0% (0/30)	18.8% (3/16)	0% (0/16)	21.4% (3/14)	0% (0/14)
Fetal NP	0% (0/10)	0% (0/10)	NA	NA	NA	NA	NA	NA
Dysplastic NP (Total)	75.0% (6/8)	50.0% (4/8)	NA	NA	NA	NA	NA	NA
High-grade	75.0% (3/4)	100% (4/4)
Low-grade	75.0% (3/4)	0% (0/4)