Article Figures & Data
Figures
- Fig. 1.
Clonogenic survival of HT1080 cells is reduced after loss of activated N-ras. Cells in log phase growth were plated and irradiated for clonogenic survival at the doses indicated. After 14–21 days, plates were stained and scored for colony formation. SG-1 (○), HT1080 cells expressing both activated and wild-type endogenous N-ras alleles. SG-2 (Δ), HT1080 expressing only the wild-type endogenous N-ras allele. SG-6 (□), HT1080 expressing the wild-type endogenous N-ras allele and an activated N-ras introduced by transfection. Bars, SD.
- Fig. 2.
Radiation-induced apoptosis after loss of activated N-ras expression. Cells in log phase were irradiated with 10 Gy and scored for apoptotic changes in nuclear morphology at 24 and 48 h after irradiation.
- Fig. 3.
Clonogenic survival of DLD-1 cells is reduced after loss of activated K-ras. Cells in log phase growth were plated and irradiated for clonogenic survival at the doses indicated. Three weeks after irradiation, plates were stained and scored for colony formation. SG-3 (○), DLD-1 cells expressing both activated and wild-type endogenous K-ras alleles. SG-4 (▴) and SG-5 (▪), DLD-1 cells expressing only the wild-type endogenous K-ras allele. Bars, SD.
- Fig. 4.
Cell proliferation in cultures of HT1080 and DLD1. Cells were plated at 2 × 105 cells/dish on day 0 and counted on the days indicated. A, SG-1 (□) and SG-2 (○) were treated with farnesyltransferase inhibitor beginning on day 2 of culture (▪, •). B, SG-3 (□) and SG-4 (○). Data represent the means of three replicate counts. Bars, SD.
Tables
- Table 1
Cell cycle distribution of log phase cultures
Cell cycle distribution was determined from flow profiles obtained on day 5 of culture (Fig. 3) <$REFLINK> by analysis of DNA content from replicate dishes of the cells used for growth curves.
Cell line G0-G1 S G2-M SG-1 46.1 28.3 25.6 SG-2 50.0 31.4 18.6 SG-3 47.2 28.7 24.1 SG-4 51.7 27.7 20.6
Volume 60, Issue 23
