Article Figures & Data
Figures
- Fig. 1.
Ms-SNuPE analysis (patient 487). Quantitative methylation analysis of two or three CpG sites in PAX6 exon 5, p16 exon 2, 5′ end of DBC, and 5′ end of TPEF using the Ms-SNuPE technique. The percent methylation represents the average of two or three sites using the following equation on PhosphoImager quantitation: (methylated)C/[(methylated)C + (unmethylated)T] × 100.
- Fig. 2.
Progressive methylation changes of four CpG islands in bladder cancer. Ps represent differences from normal controls.
- Fig. 3.
Proportion of patients with zero to four CpG islands methylated over 20% in each group of the tissues examined. A statistically significant trend (P = 0.047) is shown with increasing methylation in progressively invasive tumors.
Tables
- Table 1
Primer sequences for bisulfite PCR and Ms-SNuPE
Primers Nucleotide Sequence Bisulfite PCR PAX6 exon 5 Sense 5′-GGG AGG ATT ATT TGT AG-3′ Antisense 5′-CTT-TCC-TCA-AAT-CAC-AAC-3′ p16 exon 2 Sense 5′-GTA GGT GGG GAG GAG TTT AGT T-3′ Antisense 5′-TCT AAT AAC CAA CCA ACC CCT CC-3′ 5′ Region of DBC Sense 5′-GAA ATT TTT TA(T/C) GGT TTA GTT TTA GTT TAT T-3′ Antisense 5′-ACT AAC (A/G)AA TAA AAA AAT AAA TCC AAA TAC-3′ 5′ Region of TPEF Sense 5′-AAT TAG TTA TGG TGT TGT GG-3′ Antisense 5′-CTA AAA TAA ACT AAT CTA TAC TAA ATA C-3′ Ms-SNuPE PAX6 exon 5 17 mer 5′-GTT GAT AAA GAT ATT AT-3′ 19 mer 5′-GAG GAT TAT TTG TAG AAT T-3′ 23 mer 5′-AGT TAG TTT TAT AAT TTT TTG T-3′ p16 exon 2 15 mer 5′-GTT GGT GGT GTT GTA-3′ 18 mer 5′-AGG TTA TGA TGA TGG GTA G-3′ 21 mer 5′-TAT TAG AGG TAG TAA TTA TGT T-3′ 5′ Region of DBC 19 mer 5′-GGT TTA GTT TTA GTT TAT T-3′ 21 mer 5′-AAA GAT GTA TAA AAT ATT TTT-3′ 5′ Region of TPEF 19 mer 5′-GTT GGT TGT TGT TGT TGT T-3′ 23 mer 5′-TAG TGT AGT AGT TGG ATA TTT TG-3′ 27 mer 5′-AAT TGT TTT GGT AAG TTT AGA ATT TT-3′
Volume 60, Issue 9
