Article Figures & Data
Figures
- Fig. 1.
Amino acid sequences of mSteap and human STEAP. Shaded regions highlight conserved amino acids. The underlined regions correspond to the putative membrane-spanning domains. No NH2-terminal signal peptide is found, and NH2 and COOH termini are both predicted to be intracellular by use of web tool PSORT II.
- Fig. 2.
Northern blot analysis of mSteap and mPsca expression. Total RNA from normal prostate, TRAMP prostate tumor tissues, TRAMP-C1 cells, and TRAMP-C2 cells were analyzed by using mSteap- or mPsca-specific probes. The normal prostate lane contained 20 μg of total RNA, and the other lanes were prepared with 5 μg of total RNA. All of the RNA samples were normalized by ethidium bromide staining and subsequent analysis with an β-actin probe.
- Fig. 3.
RT-PCR analysis of mRNA expression of mSteap, mPsca, and mPsma in normal mouse tissues. DNase-treated total RNA from the indicated tissues was submitted to 35 cycles of RT-PCR amplification with mSteap-, mPsca-, and mPsma-specific primers, respectively. All of the PCR products were visualized on 2% agarose gel stained with ethidium bromide.
- Fig. 4.
RT-PCR analysis of mRNA expression of mSteap, mPsca, and mPsma in TRAMP-C cells, primary tumors, and metastatic tumors of TRAMP mice. DNase-treated total RNA was submitted to 35 cycles of RT-PCR amplification with mSteap-, mPsca-, and mPsma-specific primers, respectively. Lanes are: 1, TRAMP-C1; 2, TRAMP-C2; 3 and 4, primary prostate tumors; 5, 6, and 7, liver metastases; 8 and 9, lymph node metastases; and 10, visceral metastases. All of the PCR products were visualized on 2% agarose gel stained with ethidium bromide.
Volume 61, Issue 15
