MAGE-A Gene Expression Pattern in Primary Breast Cancer

DOI: Published September 2001

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Fig. 1.
Analysis of three breast cancer patients for MAGE-A expression. In two patients (PC and FrS) a multi-MAGE-A product was found.
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Fig. 2.
Determination of the MAGE-A expression pattern by restriction endonuclease treatment. The multi-MAGE-A product was digested with two sets of restriction endonucleases (see Table 1 <$REFLINK> ).

Table 1

Restriction endonuclease mixtures, multi-MAGE-A-products, and restriction fragments for each MAGE-A gene tested

Assay no.	Restriction endonuclease mixture	MAGE gene	PCR product length (bp)	Fragment length (bp)
	Bcl I	A1	893	106, 787
1	EcoRI	A3	914	167, 747
	Eco47 III	A4	917	375, 542

	Sph I	A2	914	21, 22, 151, 720
2	Afl III	A6	914	22, 172, 282, 438
	Avi II	A12	914	22, 84, 172, 636

Table 2

Expression pattern of MAGE-A1, -2, -3, -4, -6, and -12 in 18 MAGE-A-positive primary breast carcinomas

Patient	MAGE-A gene expression						Age	T	N	M	Grade	Histology	ER	PR	HER-2^a	uPA^a	PAI-1^a	Ki-67^a
Patient	1	2	3	4	6	12	Age	T	N	M	Grade	Histology	ER	PR	HER-2^a	uPA^a	PAI-1^a	Ki-67^a
SB	+	−	−	−	−	−	64	T₂	N₂	M_x	G2	Medullary	+	+	NA	NA	NA	NA
DH	−	+	−	−	−	−	58	T₁	N₀	M₀	G2	Mucinous	+	+	High	NA	NA	NA
FrM	−	+	−	−	−	−	72	T₄	N₂	M₁	G3	Ductal	+	−	High	NA	NA	High
ME	−	+	−	−	−	−	70	T₄	N₀	M_x	G3	Ductal	+	+	NA	High	Norm	NA
SE	−	−	−	+	−	−	72	T₂	N₁	M₀	G3	Ductal/DCIS	−	+	High	NA	NA	High
DJ	−	−	−	−	+	−	57	T₁	N₁	M₀	G2	Ductal/DCIS	+	−	Interm	Norm	High	Interm
SR	−	−	−	−	+	−	70	T₂	N₀	M₀	G2	Ductal	+	+	Low	Norm	Norm	NA
CG	+	+	−	−	−	−	46	T₂	N₁	M₀	G3	Ductal	+	−	High	Norm	Norm	High
PC	+	+	−	−	−	−	63	T₁	N₀	M₀	G3	Ductal	−	−	Interm	High	Norm	High
FrS	−	+	−	+	−	−	35	T₃	N₀	M₁	G3	Sarcomatoid	−	−	High	NA	NA	High
NH	−	−	+	−	+	−	59	T₂	N₀	M₀	NA	Ductal/lobul	+	+	NA	High	High	NA
MM	−	+	−	+	+	−	69	T₂	N₁	M₀	G2	Ductal	+	+	NA	High	High	NA
UL	−	+	+	+	+	+	44	T₁	N₁	M₀	G3	Ductal/DCIS	+	−	High	High	High	High
WH	−	+	+	+	+	+	50	T₂	N₀	M₀	G2	Ductal	+	+	Neg	High	High	High
DR	−	+	+	+	+	+	53	T₁	N₁	M₀	G2	Ductal	+	+	Interm	Norm	Norm	Interm
KT	−	+	+	+	+	+	38	T₁	N₀	M₀	G2	Ductal	+	+	High	High	High	High
BI	−	+	+	+	+	+	56	T₂	N₀	M₀	G3	Ductal	−	+	Neg	High	High	High
MC	+	+	+	+	+	+	65	T₁	N₀	M_x	G2	Ductal	−	−	High	NA	NA	High

Table 3

Group-specific proportions (%) of primary breast tumors expressing MAGE-A1, 2, 3, 4, 6, and 12 in relation to clinical and pathological factors

Clinicopathological factor	Variation		≥1 MAGE-A % positive	MAGE A1 % positive	MAGE A2 % positive	MAGE A3 % positive	MAGE A4 % positive	MAGE A6 % positive	MAGE A12 % positive
Age	>50	(n = 51)	25	6	16	8	10	14	6
	=50	(n = 16)	31	6	31	19	25	19	19
Histologic type	Ductal	(n = 41)	34	7	27	15	20	22	15
	Lobular	(n = 15)	0^b	0	0	0	0	0	0
	Other	(n = 11)	36	9	18	9	9	9	9
Tumor size	T₁	(n = 21)	33	9	29	19	19	24	19
	T₂	(n = 40)	20	5	10	8	10	13	5
	T_3–4	(n = 6)	50	0	50^b	0	17	0	0
Lymph node metastasis	N₀	(n = 39)	26	5	21	13	13	15	10
	N₊	(n = 26)	31	8	19	8	15	15	8
Distant metastases	M₀	(n = 36)	36	6	25	17	19	25	14
	M₁	(n = 4)	50	0	50	0	25	0	0
Grade	G1	(n = 8)	0	0	0	0	0	0	0
	G2	(n = 30)	30	7	20	13	17	23	7
	G3	(n = 28)	29	7	25	7	14	7	14
ER	Positive	(n = 46)	28	4	20	11	11	17	9
	Negative	(n = 20)	25	10	20	10	20	10	10
PR	Positive	(n = 41)	27	2	17	12	15	17	10
	Negative	(n = 25)	28	12	24	8	12	12	8
HER-2	Low/Int^a	(n = 22)	27	5	18	14	14	23	14
	High	(n = 28)	29	7	25	11	18	11	11
uPA	Normal	(n = 16)	25	6	13	6	6	19	6
	High	(n = 16)	50	6	44	31	31	38	25
PAI-1	Normal	(n = 19)	26	11	21	26	5	11	5
	High	(n = 13)	54	0	39	8	39	54^b	31
Ki-67	Low/Int	(n = 15)	13	0	7	7	7	13	7
	High	(n = 29)	35	10	31	17	24	17	17