Article Figures & Data
Figures
- Fig. 1.
Changes in survivin expression after induction of wt-APC expression in HT29 cells. The data shows that induction of wt-APC decreases expression of survivin mRNA and protein in recombinant HT-29 cells (HT-29-APC), but not in control cells (HT-29-Gal), in which wt-APC is not induced. A, survivin mRNA (363 bp) levels were assessed by RT-PCR in HT-29-APC cells and HT-29-Gal cells at the indicated times after ZnCl2-induced expression of wt-APC. β-actin mRNA (564 bp) levels are shown as an internal control. B, survivin protein (Mr 16,500) levels were assessed by Western blots in the same cell lines. Tubulin protein (Mr 55,000) levels are shown as an internal control.
- Fig. 2.
Changes in survivin expression in HT-29 cells that were transfected with a dominant-negative TCF-4 gene construct. A, HT-29 cells transiently transfected with varying amounts of plasmid DNA (0, 1.0, and 2.0 μg) encoding a dominant-negative TCF-4 gene construct. These data show that transiently blocking TCF-4 mediated activation of gene transcription decreases survivin expression. Survivin mRNA levels were evaluated by RT-PCR for two different protocols (25 or 28 cycles). β-actin levels are shown as an internal control. B, HT-29 cells stably transfected with plasmid DNA encoding a dominant-negative TCF-4 gene construct (−) or the empty plasmid (+). These data show that stably blocking TCF-4-mediated activation of gene transcription decreases survivin expression. Survivin mRNA levels were evaluated by RT-PCR for two different protocols (25 or 28 cycles). C, survivin protein levels in HT-29 cells stably transfected with the dominant-negative TCF-4 gene construct were evaluated by Western blots. Tubulin levels are shown as an internal control.
- Fig. 3.
Survivin expression in normal human colonic mucosa. A, survivin mRNA expression in different sections of microdissected normal crypts isolated from 10 patients. Using RT-PCR, we showed that survivin is preferentially expressed in the bottom (B) crypt section as compared with the middle (M) and top (T) sections of normal human crypts. β-actin levels are shown as an internal control. B and C, survivin immunoreactivity in normal human colonic mucosa. Staining is most prominent at the base of the crypt, considerably lower in the middle region, and not present at the crypt top. ×100 (B). Outlined area in B is shown at higher magnification (×400) in C.
Volume 61, Issue 24
