Erratum
In the article by J-L. Fang et al., entitled, “Characterization of Benzo(a)pyrene-trans-7,8-dihydrodiol Glucuronidation by Human Tissue Microsomes and Overexpressed UDP-glucuronosyltransferase Enzymes,” which appeared in the , Figure 5 ⇓ attempts to demonstrate that human aerodigestive tract tissue microsomes exhibit significant glucuronidation activity against racemic benzo(a)pyrene-trans-7,8-dihydrodiol (BPD). It also attempts to show that differences in the BPD-glucuronide diastereomer pattern observed for aerodigestive tract tissue microsomes is distinctly different from that observed for microsomes prepared from human liver specimens (Figure 2 of the article). These data are consistent with the BPD-glucuronide diastereomer formation patterns observed with the different UDP-glucuronosyltransferase enzymes (Figure 4 of the article) expressed in the different tissues. The BPD glucuronide HPLC peaks in Figure 5 ⇓ were not clear. The correct Figure 5 ⇓ and corresponding legend appear to the right.
HPLC analysis of BPD glucuronide formation by human laryngeal and esophageal microsomes. Shown are the UV traces of HPLC separation of metabolites formed in assays using human microsomes (1 mg of protein) incubated with 2 mm racemic BPD and 4 mm UDPGA as described in “Materials and Methods.” Aand B, esophageal microsomes; Cand D, laryngeal microsomes. B and D, assays including β-glucuronidase as described in “Materials and Methods.” BPD glucuronide conjugates are indicated.
- ©2002 American Association for Cancer Research.
Volume 62, Issue 11
