HeLa cells were transfected with GFP-Survivin, simultaneously fixed and extracted, and analyzed for GFP labeling by confocal fluorescence microscopy. DNA was stained in red. GFP-Survivin localizes to kinetochores of metaphase chromosomes and decorates the entire length of mitotic spindle microtubules at metaphase. For details, see the article by Giodini et al. on page 2462 of this issue.