Article Figures & Data
Figures
- Fig. 1.
Effect of 1-h and 24-h resveratrol treatment on [3H]thymidine incorporation into DNA of LNCaP cells. Exponentially growing cells were treated with various concentrations of resveratrol for either 1 h (▪) or 24 h (•) as described in “Materials and Methods.” They were then pulse-labeled with [3H]thymidine for 30 min, and radioactivity incorporated into DNA was measured.
- Fig. 2.
Effect of resveratrol on [3H]thymidine and [5-3H]uridine incorporation into LNCaP cells. Cells treated with resveratrol were pulse-labeled with [3H]thymidine (•) or [5-3H]uridine (▪) and were processed for measuring radioactivity incorporated into nucleic acids as described in “Materials and Methods.”
- Fig. 3.
Comparison of resveratrol effect on [3H]thymidine incorporation in androgen-sensitive (LNCaP), androgen-independent (DU145) prostate epithelial cells, and NIH 3T3 fibroblast cells. LNCaP (•) and DU145 (▪) prostate epithelial cells, and NIH 3T3 fibroblast cells (▴) were plated similarly and treated with resveratrol for 24 h, and [3H]thymidine incorporation into DNA was measured as described in “Materials and Methods.”
- Fig. 4.
Cell cycle analysis of exponentially growing LNCaP cells treated with resveratrol. Exponentially growing LNCaP cells were treated with diluent (control) or with 10 μm or 20 μm resveratrol as described in “Materials and Methods.” After 24 h, the cells were prepared for flow cytometry analysis as described in “Materials and Methods.” The table shows the percentage of G1, S phase, and G2-M populations of cells under each treatment condition. These data are representative of at least three independently repeated experiments.
- Fig. 5.
Effect of resveratrol on the nuclear cell cycle regulatory proteins in LNCaP cells. Exponentially growing LNCaP cells were treated with the indicated concentrations of resveratrol for 24 h. Nuclear lysates were prepared and immunoblot analysis using monoclonal antibodies to detect p21Cip1, p27Kip1, Cdk2, and cyclins A, B, and E was performed as described in “Materials and Methods.” Profiles of cell cycle regulatory proteins are representative of at least three independently repeated experiments.
- Fig. 6.
Effect of resveratrol on Cdk2 activity associated with cyclin A and cyclin E in LNCaP cells. Exponentially growing LNCaP cells were treated with 10 or 25 μm resveratrol. Cyclin A- and cyclin E-specific immunoprecipitates were prepared from nuclear lysates, and Cdk2 activity was measured by histone H1 kinase assay (A), and p21Cip1, p27Kip1, cyclin A, and cyclin E were measured by Western blot analysis (B) in each of the immunoprecipitates as described in “Materials and Methods.” Immunoprecipitates recovered from equal amount of protein (25 μg) in individual nuclear lysates were used in these assays. Results are representative of at least two independently repeated experiments.
Volume 62, Issue 9
