Article Figures & Data
Figures
- Fig. 1.
2DG-induced cytotoxicity and radiosensitization in HeLa cells. A, clonogenic survival curves using HeLa cells treated with 4, 6, or 10 mm 2DG for 4, 24, 48, or 72 h. Surviving fractions were normalized to untreated controls. A linear regression model was used to assess the correlations between surviving fraction and 2DG time/dose, and these parameters were found to correlate linearly (R2 = 0.98). B, clonogenic cell survival curves using HeLa cells treated with 4 or 6 mm 2DG for 4 or 16 h before exposure to IR at 2, 4, or 8 Gy. Survival data were normalized to unirradiated controls from each respective treatment group.
- Fig. 2.
2DG-induced cytotoxicity is inhibited by NAC. Clonogenic cell survival of HeLa cells treated with 4, 6, or 8 mm 2DG for 4 (A) or 8 (B) h with (+) or without (−) treatment with 30 mm NAC for 1 h before and during 2DG exposure. Clonogenic cell survival data were normalized to untreated controls, and errors represent ±1 SD. Statistical relationships between treated and untreated cells were determined by two-sample, two-sided t tests comparing the means obtained ± NAC at each dose of 2DG. ∗, statistical significance at the 0.05 level.
- Fig. 3.
2DG-induced radiosensitization is inhibited by NAC. Clonogenic survival of HeLa cells treated with 6 mm 2DG for 16 h before exposure to 4 or 8 Gy of IR with (+) or without (−) treatment with 30 mm NAC for 1 h before and during 2DG exposure. Clonogenic cell survival data were normalized to unirradiated controls from each respective treatment group, and errors represent ±1 SD. ∗, statistically significant comparisons of 2DG-treated cells with 2DG + NAC-treated cells.
- Fig. 4.
The effect of 2DG and NAC on glutathione levels in control and irradiated HeLa cells. HeLa cells were treated for 8 h with 6 mm 2DG in the presence and absence of 30 mm NAC, irradiated, and harvested for glutathione analysis using the spectrophotometric recycling assay. Errors represent ±1 SD of n = 3 samples. Statistical relationships between groups were determined by two-sample, two-sided t tests. ∗, statistically significant comparisons of 2DG alone-treated cells to control. ∗∗, statistically significant comparisons of 2DG + NAC-treated cells to 2DG alone-treated cells.
- Fig. 5.
The effect of 2DG and NAC on thiol levels in control and irradiated HeLa cells. HeLa cells were treated for 8 h with 6 mm 2DG in the presence and absence of 30 mm NAC, irradiated, and harvested for high-performance liquid chromatography thiol analysis. Errors represent ±1 SD of n = 3 samples. Statistical relationships between groups were determined by two-sample, two-sided t tests. ∗, statistically significant comparisons of 2DG alone-treated cells to control. ∗∗, statistically significant comparisons of 2DG + NAC-treated cells to 2DG alone-treated cells.
- Fig. 6.
Enhanced susceptibility of v-Fos-transformed fibroblasts to 2DG-induced cytotoxicity. Clonogenic cell survival of 208F (immortalized rat fibroblasts) and FBJ/R (v-Fos-transformed 208F cells) treated with 6 or 10 mm 2DG for 4, 8, 16, or 24 h. Clonogenic cell survival data were normalized to untreated controls, and errors represent ±1 SD. ∗, statistically significant comparisons of FBJ cells to the identically treated 208F cells.
- Fig. 7.
2DG-induced radiosensitization in oncogene-transformed fibroblasts. A, clonogenic cell survival of 208F (immortalized rat fibroblasts) and FBJ/R (v-Fos-transformed 208F cells) treated with 2, 4, 6, 8, or 10 Gy of IR. Surviving fractions are normalized to sham-treated controls from each cell line. B, clonogenic cell survival of 208F and FBJ/R treated with 6 mm 2DG for 4 or 8 h before and during treatment with 2 Gy IR. Survival data were normalized to unirradiated controls from each respective group, and errors represent ±1 SD. Statistical relationships between identically treated samples of 208F and FBJ/R cells were determined by two-sample, two-sided t tests. ∗, statistically significant comparisons.
- Fig. 8.
2DG-induced radiosensitization in oncogene-transformed fibroblasts is inhibited by treatment with NAC. Clonogenic cell survival of 208F (immortalized rat fibroblasts) and FBJ/R (v-Fos-transformed 208F cells) treated with 2 Gy of IR in the presence and absence of 6 mm 2DG and 30 mm NAC. Surviving fractions are normalized to unirradiated controls from each respective drug-treated group, and errors represent ±1 SD. Statistical relationships between identically treated samples of 208F and FBJ/R cells were determined by two-sample, two-sided t tests. ∗, statistically significant comparisons.
Volume 63, Issue 12
