Article Figures & Data
Figures
- Fig. 5.
Basic residues within DEL are required for Syk(L) nuclear localization. A, immunohistochemical examination of Syk(L) and Syk(S) subcellular localization. Pooled MDA-MB-435S stable lines that expressed cDNAs of SYK(L), SYK(S), or SYK(L) with replaced basic residues (M1, M2, and M3) were fixed. Syk-immunoreactive proteins were examined by N-19 polyclonal antibody as described in “Materials and Methods.” After immunodetection, cells were counterstained with hematoxylin. Neo control was used to verify the specificity of Syk immunostaining (background). B, the above MDA-MB-435S stable lines were subjected to nuclear (N) and cytosolic (C) fractionation followed by SDS-PAGE and immunoblotting with anti-Syk, Oct-1, or actin antisera. Total cell lysates (T) were run in parallel.
- Fig. 6.
Subcellular localization of GFP fusion proteins. A, COS7 cells were transfected with the parental vector, pEGFP-DEL, pEGFP-IDB(L), or pEGFP-IDB(L)-M. Cells were fixed and the nucleus stained with DAPI. The localization of GFP (top panel) and DAPI-stained nucleus (bottom panel) was examined by fluorescence microscopy. B, localization of GFP fusion protein was scored, and an average of three independent experiments was plotted. Diffuse GFP distribution throughout cells was scored as cytoplasmic (blue bars). Nuclear localization was determined when nuclear GFP fusion protein was prominent (red bars).
Volume 63, Issue 20
