PhIP-DNA adducts as a biomarker for prostate carcinogenesis

Abstract

2057

2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is the most abundant heterocyclic amine in the human diet, and is formed during the cooking of meat. PhiP-DNA adducts provide a measure of chemical-specific genetic damage that has been associated with increased risk of cancer. This study examined the relationship between PhiP-DNA adducts measured by immunohistochemistry in nontumorous and tumorous prostatic epithelial cells. Study subjects were 125 men who underwent prostatectomy and were part of the Henry Ford Health System, in Detroit, Michigan. . The mean level of PhiP-DNA adducts in prostatic epithelial nontumor cells was significantly higher than the mean level of PhiP-DNA adducts in tumor cells (0.055 units) (p<0.0001). While tumor stage was not associated with PhIP-DNA adducts, adduct levels in both the nontumor (p=0.0002) and tumor (p=0.005) cells had a statistically significant positive association with Gleason grade. Only PhIP-DNA adducts in normal cells were significantly associated with prostate-specific antigen (PSA) levels (p<0.0001). Our findings suggest that PhiP-DNA adducts may be a biomarker for prostate carcinogenesis.