Article Figures & Data
Figures
- Figure 1.
Survival curves of female Xpc−/−, Xpa−/− and their wild-type control cohorts. C57BL/6 1 is the control cohort for Xpa−/−. C57BL/6 2 is the control cohort for Xpc−/−. Median survival of the cohorts: C57BL/6 1, 103 wk (light green); C57BL/6 2, 102.5 wk (dark green); Xpc−/−, 94 wk (P = 0.0023; red); Xpa−/−, 94.5 wk (P = 0.6023; blue). For further details, see Materials and Methods.
- Figure 2.
Hprt mutant frequencies including SDs of Xpc−/−, Xpc+/− and their wild-type controls at ages 13 and 52 wk in spleen. The numbers of biological replicas are between 4 and 10. For further details, see Materials and Methods.
- Figure 3.
LacZ mutant frequencies including SDs of Xpc−/−, Xpa−/− and their wild-type controls at ages 13, 52, and 78 wk in spleen (A), liver (B), and lung (C). The numbers of biological replicas are between 5 and 6. For further details, see Materials and Methods.
- Figure 4.
Survival of MEFs including SDs of Xpc−/−, Xpa−/− and their wild-type controls under different levels of oxygen pressure. Cells at the first passage were all cultured in 3% oxygen (A); subsequent passages were cultured in 3% (B) or 20% (C) oxygen.
Tables
- Table 1.
Tumor incidences and P values for difference between mutant and its wild-type group
C57BL/6J 1 Xpa−/− P C57BL/6J 2 Xpc−/− P No. animals (absolute) 40 31 29 38 All organs Tumor-bearing animals 30 (75) 21 (68) 24 (83) 23 (61) Liver Hepatocellular tumor 0 (0) 3 (10) 0.0002 2 (7) 5 (13) * Lung Bronchioloalveolar tumor 0 (0) 2 (6) 1 (3) 6 (16) 0.02 Pituitary Pars distalis adenoma 20 (50) 8 (26) * 20 (70) 9 (24) 0.01 All organs Pituitary adenomas excluded 18 (45) 19 (61) 0.001 21 (72) 23 (61) † -
NOTE: Data are absolute values with percentages in brackets. Statistics: poly-k test (poly-3 test and Peto test generally gave similar significances, although exact P values may differ).
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↵* Approaches significance with poly-3 test only (P = 0.08 for Xpc−/− liver and P = 0.054 for Xpa−/− pituitary).
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↵† Not reaching significance with poly-k test, but P = 0.05 (positive trend) by the Peto test in this case.
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- Table 2.
P values of mutant frequency comparisons between genotypes, depicted per age per tissue
Spleen 13 wk 52 wk 78 wk Liver 13 wk 52 wk 78 wk Lung 13 wk 52 wk 78 wk Xpc vs WT 0.016 0.012 Xpc vs WT 0.002 Xpc vs WT 0.04 0.00003 0.0001 Xpc vs Xpa 0.0098 0.007 Xpc vs Xpa Xpc vs Xpa 0.02 0.0005 Xpa vs WT Xpa vs WT 0.04 0.002 Xpa vs WT 0.003 0.003 -
NOTE: Open cells represent nonsignificant (P > 0.05) differences.
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Volume 68, Issue 5
