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Molecular and Cellular Pathobiology

BRAF Inactivation Drives Aneuploidy by Deregulating CRAF

Tamihiro Kamata, Jahan Hussain, Susan Giblett, Robert Hayward, Richard Marais and Catrin Pritchard
Tamihiro Kamata
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Jahan Hussain
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Susan Giblett
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Robert Hayward
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Richard Marais
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Catrin Pritchard
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DOI: 10.1158/0008-5472.CAN-10-0603 Published November 2010
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Abstract

Aspartate-594 is the third most common BRAF residue mutated in human cancer. Mutants of this residue are kinase inactive, and the mechanism(s) by which they contribute to cancer has remained perplexing. Using a conditional knock-in mouse model, we show that the D594ABraf mutant does not drive tumor development per se but is able to induce aneuploidy in murine splenocytes and mouse embryonic fibroblasts and contributes to immortalization through the propagation of aneuploid cells. D594ABraf lacks kinase activity but induces the related gene product Craf as well as the mitogen-activated protein/extracellular signal-regulated kinase (ERK) kinase (MEK)/ERK pathway. Here, we show that the aneuploid phenotype is dependent on Craf. Treatment with the MEK inhibitor U0126 did not attenuate the emergence of aneuploidy but prevented the growth of aneuploid cells. These results provide a previously unidentified link between Craf and chromosomal stability, with important implications for our understanding of the development of cancers with driver mutations that hyperactivate Craf. Cancer Res; 70(21); 8475–86. ©2010 AACR.

Footnotes

  • Note: Supplementary data for this article are available at Cancer Research Online (http://cancerres.aacrjournals.org/).

  • Received February 18, 2010.
  • Revision received June 23, 2010.
  • Accepted August 31, 2010.
  • ©2010 American Association for Cancer Research.
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Cancer Research: 70 (21)
November 2010
Volume 70, Issue 21
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BRAF Inactivation Drives Aneuploidy by Deregulating CRAF
Tamihiro Kamata, Jahan Hussain, Susan Giblett, Robert Hayward, Richard Marais and Catrin Pritchard
Cancer Res November 1 2010 (70) (21) 8475-8486; DOI: 10.1158/0008-5472.CAN-10-0603

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BRAF Inactivation Drives Aneuploidy by Deregulating CRAF
Tamihiro Kamata, Jahan Hussain, Susan Giblett, Robert Hayward, Richard Marais and Catrin Pritchard
Cancer Res November 1 2010 (70) (21) 8475-8486; DOI: 10.1158/0008-5472.CAN-10-0603
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