Skip to main content
  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

AACR logo

  • Register
  • Log in
  • My Cart
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Meeting Abstracts
    • Collections
      • COVID-19 & Cancer Resource Center
      • Focus on Computer Resources
      • Highly Cited Collection
      • Editors' Picks
      • "Best of" Collection
  • For Authors
    • Information for Authors
    • Author Services
    • Early Career Award
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citations
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
  • COVID-19
  • Webinars
  • Search More

    Advanced Search

  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in
  • My Cart

Search

  • Advanced search
Cancer Research
Cancer Research
  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Meeting Abstracts
    • Collections
      • COVID-19 & Cancer Resource Center
      • Focus on Computer Resources
      • Highly Cited Collection
      • Editors' Picks
      • "Best of" Collection
  • For Authors
    • Information for Authors
    • Author Services
    • Early Career Award
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citations
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
  • COVID-19
  • Webinars
  • Search More

    Advanced Search

Understanding the Origins of Colorectal Neoplasia

Abstract A04: Chromosomal aberrations implicated in colorectal adenoma to carcinoma progression as markers of high-risk colorectal adenomas

Beatriz Carvalho, Begoña Diosdado, Jochim S. Terhaar Sive Droste, Anne S. Bolijn, Meike de Wit, Myrthe van Burink, Remond JA Fijneman, Nicole CT van Grieken and Gerrit A. Meijer
Beatriz Carvalho
1The Netherlands Cancer Institute, Amsterdam, The Netherlands,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Begoña Diosdado
1The Netherlands Cancer Institute, Amsterdam, The Netherlands,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jochim S. Terhaar Sive Droste
2Jeroen Bosch Hospital, Den Bosch, The Netherlands,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anne S. Bolijn
1The Netherlands Cancer Institute, Amsterdam, The Netherlands,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Meike de Wit
1The Netherlands Cancer Institute, Amsterdam, The Netherlands,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Myrthe van Burink
3VU University Medical Center, Amsterdam, The Netherlands.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Remond JA Fijneman
1The Netherlands Cancer Institute, Amsterdam, The Netherlands,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nicole CT van Grieken
3VU University Medical Center, Amsterdam, The Netherlands.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gerrit A. Meijer
1The Netherlands Cancer Institute, Amsterdam, The Netherlands,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1158/1538-7445.CRC16-A04 Published February 2017
  • Article
  • Info & Metrics
Loading
Abstracts: AACR Special Conference: Colorectal Cancer: From Initiation to Outcomes; September 17-20, 2016; Tampa, FL

Abstract

Background: Colorectal adenomas are precursor lesions of colorectal cancer. About 5% of colorectal adenomas are estimated to progress to colorectal cancer (CRC). The currently used histo-pathological characteristics to identify adenomas at risk of malignant progression, i.e. scoring of size ≥ 10mm, villous component or high grade dysplasia, are not sufficiently sensitive and specific and prone to inter-observer variability. Integrating molecular markers reflecting the underlying biology of CRC into the current classification system is expected to better characterize adenomas at high-risk of progression.

Aim: Here we compared the prevalence of genetic changes, in particular DNA copy number changes, between advanced and non-advanced adenomas.

Methods: Formalin-fixed paraffin-embedded tissue samples from 121 patients (mean age 65.4±11.6, 52.9% male) with colorectal adenomas (63 non-advanced adenomas and 58 advanced adenomas) were retrospectively collected from the archives of the department of pathology of the VU - University medical center (VUmc), Amsterdam, the Netherlands. DNA was extracted and DNA copy number analysis was performed using Multiplex Ligation-dependent Probe Amplification (MLPA), focusing on 7 chromosomal regions previously associated with adenoma-to-carcinoma progression, namely 8p, 15q, 17p and 18q loss and 8q,13q and 20q gain (1). Comparison of DNA copy number aberrations between sub-groups was done using a Chi-square test, or Fisher's exact test when appropriate. For multivariate analysis logistic regression was used. Data analysis was performed using SPSS version 22 (IBM SPSS Statistics) and p-values below 0.05 were considered to be statistically significant (using two-sided tests).

Results: Of the adenomas analyzed 23.1% (28/121) showed at least one gain or loss of the investigated chromosomal regions. Two or more chromosomal aberrations were present in 22.4 % (13/58) of the advanced adenomas and 1.6% (1/63) of the non-advanced adenomas (p=0,001). Since advanced colorectal adenomas are defined based on their histological characteristics ((tubulo)villous component and/or high degree dysplasia and/or ≥ 10mm), we searched for associations of these histological features with the analyzed chromosomal aberrations (CAE's). Multivariate analysis showed that only gains of 13q and 20q were significantly associated with adenomas ≥ 10mm (p=0.01 and p=0.003, respectively) and losses of 18q were significantly associated with high-grade dysplasia (p=0.04).

Conclusion: Our data indicate that DNA copy number alterations are not as frequent as expected in advanced adenomas and are also found in non-advanced and diminutive lesions. Combining histological and molecular features in adenoma follow-up studies could increase the knowledge on malignant potential of adenomas.

(1) Hermsen et al, Gastroenterology, 2002; 123:1109-1119

Citation Format: Beatriz Carvalho, Begoña Diosdado, Jochim S. Terhaar Sive Droste, Anne S. Bolijn, Meike de Wit, Myrthe van Burink, Remond JA Fijneman, Nicole CT van Grieken, Gerrit A. Meijer. Chromosomal aberrations implicated in colorectal adenoma to carcinoma progression as markers of high-risk colorectal adenomas. [abstract]. In: Proceedings of the AACR Special Conference on Colorectal Cancer: From Initiation to Outcomes; 2016 Sep 17-20; Tampa, FL. Philadelphia (PA): AACR; Cancer Res 2017;77(3 Suppl):Abstract nr A04.

  • ©2017 American Association for Cancer Research.
Previous
Back to top
Cancer Research: 77 (3 Supplement)
February 2017
Volume 77, Issue 3 Supplement
  • Table of Contents

Sign up for alerts

Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Abstract A04: Chromosomal aberrations implicated in colorectal adenoma to carcinoma progression as markers of high-risk colorectal adenomas
(Your Name) has forwarded a page to you from Cancer Research
(Your Name) thought you would be interested in this article in Cancer Research.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Abstract A04: Chromosomal aberrations implicated in colorectal adenoma to carcinoma progression as markers of high-risk colorectal adenomas
Beatriz Carvalho, Begoña Diosdado, Jochim S. Terhaar Sive Droste, Anne S. Bolijn, Meike de Wit, Myrthe van Burink, Remond JA Fijneman, Nicole CT van Grieken and Gerrit A. Meijer
Cancer Res February 1 2017 (77) (3 Supplement) A04; DOI: 10.1158/1538-7445.CRC16-A04

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Abstract A04: Chromosomal aberrations implicated in colorectal adenoma to carcinoma progression as markers of high-risk colorectal adenomas
Beatriz Carvalho, Begoña Diosdado, Jochim S. Terhaar Sive Droste, Anne S. Bolijn, Meike de Wit, Myrthe van Burink, Remond JA Fijneman, Nicole CT van Grieken and Gerrit A. Meijer
Cancer Res February 1 2017 (77) (3 Supplement) A04; DOI: 10.1158/1538-7445.CRC16-A04
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
Advertisement

Related Articles

Cited By...

More in this TOC Section

Understanding the Origins of Colorectal Neoplasia

  • Abstract A07: Detection of structural variants and recurrent breakpoint genes in colorectal adenoma-to-carcinoma progression
  • Abstract PR02: Epigenetic and molecular drivers of inflammation-driven colorectal cancer
  • Abstract IA04: The carcinogenic potential of bacterial biofilms
Show more Understanding the Origins of Colorectal Neoplasia

Understanding the Origins of Colorectal Neoplasia: Poster Presentations - Proffered Abstracts

  • Abstract A12: Contribution of the transcription factor STAT2 in the promotion and progression of colorectal cancer
  • Abstract A15: Progression of colorectal cancer through epidermal growth factor receptor (EGFR)-independent mechanisms
  • Abstract A08: Extensive subclonal mutations in human colorectal cancers detected by Duplex Sequencing
Show more Understanding the Origins of Colorectal Neoplasia: Poster Presentations - Proffered Abstracts
  • Home
  • Alerts
  • Feedback
  • Privacy Policy
Facebook  Twitter  LinkedIn  YouTube  RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Meeting Abstracts

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians

About Cancer Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2021 by the American Association for Cancer Research.

Cancer Research Online ISSN: 1538-7445
Cancer Research Print ISSN: 0008-5472
Journal of Cancer Research ISSN: 0099-7013
American Journal of Cancer ISSN: 0099-7374

Advertisement