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Convergence and Technologies

Targeting the Temporal Dynamics of Hypoxia-Induced Tumor-Secreted Factors Halts Tumor Migration

Manjulata Singh, Xiao-Jun Tian, Vera S. Donnenberg, Alan M. Watson, JingYu Zhang, Laura P. Stabile, Simon C. Watkins, Jianhua Xing and Shilpa Sant
Manjulata Singh
Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, Pennsylvania.
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Xiao-Jun Tian
Department of Computational and Systems Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania. School of Biological and Health Systems Engineering, Arizona State University, Tempe, Arizona.
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Vera S. Donnenberg
Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, Pennsylvania. Department of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, McGowan Institute for Regenerative Medicine, and UPMC-Hillman Cancer Center, University of Pittsburgh, Pittsburgh, Pennsylvania.
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  • ORCID record for Vera S. Donnenberg
Alan M. Watson
Center for Biologic Imaging, Center for Vaccine Research, and Department of Immunology, University of Pittsburgh, Pittsburgh, Pennsylvania.
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JingYu Zhang
Department of Computational and Systems Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania.
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Laura P. Stabile
Department of Pharmacology & Chemical Biology, UPMC-Hillman Cancer Center, Pittsburgh, Pennsylvania.
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Simon C. Watkins
Center for Biologic Imaging and the Department of Cellular Biology, University of Pittsburgh, Pittsburgh, Pennsylvania.
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Jianhua Xing
Department of Computational and Systems Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania. UPMC-Hillman Cancer Center, University of Pittsburgh, Pittsburgh, Pennsylvania.
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  • For correspondence: shs149@pitt.edu xing1@pitt.edu
Shilpa Sant
Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, Pennsylvania. UPMC-Hillman Cancer Center, University of Pittsburgh, Pittsburgh, Pennsylvania. Department of Bioengineering, Swanson School of Engineering, and McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania.
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  • For correspondence: shs149@pitt.edu xing1@pitt.edu
DOI: 10.1158/0008-5472.CAN-18-3151 Published June 2019
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Abstract

Targeting microenvironmental factors that foster migratory cell phenotypes is a promising strategy for halting tumor migration. However, lack of mechanistic understanding of the emergence of migratory phenotypes impedes pharmaceutical drug development. Using our three-dimensional microtumor model with tight control over tumor size, we recapitulated the tumor size–induced hypoxic microenvironment and emergence of migratory phenotypes in microtumors from epithelial breast cells and patient-derived primary metastatic breast cancer cells, mesothelioma cells, and lung cancer xenograft cells. The microtumor models from various patient-derived tumor cells and patient-derived xenograft cells revealed upregulation of tumor-secreted factors, including matrix metalloproteinase-9 (MMP9), fibronectin (FN), and soluble E-cadherin, consistent with clinically reported elevated levels of FN and MMP9 in patient breast tumors compared with healthy mammary glands. Secreted factors in the conditioned media of large microtumors induced a migratory phenotype in nonhypoxic, nonmigratory small microtumors. Subsequent mathematical analyses identified a two-stage microtumor progression and migration mechanism whereby hypoxia induces a migratory phenotype in the initialization stage, which then becomes self-sustained through a positive feedback loop established among the tumor-secreted factors. Computational and experimental studies showed that inhibition of tumor-secreted factors effectively halts microtumor migration despite tumor-to-tumor variation in migration kinetics, while inhibition of hypoxia is effective only within a time window and is compromised by tumor-to-tumor variation, supporting our notion that hypoxia initiates migratory phenotypes but does not sustain it. In summary, we show that targeting temporal dynamics of evolving microenvironments, especially tumor-secreted factors during tumor progression, can halt tumor migration.

Significance: This study uses state-of-the-art three-dimensional microtumor models and computational approaches to highlight the temporal dynamics of tumor-secreted microenvironmental factors in inducing tumor migration.

Footnotes

  • Note: Supplementary data for this article are available at Cancer Research Online (http://cancerres.aacrjournals.org/).

  • Cancer Res 2019;79:2962–77

  • Received October 16, 2018.
  • Revision received February 1, 2019.
  • Accepted March 29, 2019.
  • Published first April 5, 2019.
  • ©2019 American Association for Cancer Research.
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Cancer Research: 79 (11)
June 2019
Volume 79, Issue 11
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Targeting the Temporal Dynamics of Hypoxia-Induced Tumor-Secreted Factors Halts Tumor Migration
Manjulata Singh, Xiao-Jun Tian, Vera S. Donnenberg, Alan M. Watson, JingYu Zhang, Laura P. Stabile, Simon C. Watkins, Jianhua Xing and Shilpa Sant
Cancer Res June 1 2019 (79) (11) 2962-2977; DOI: 10.1158/0008-5472.CAN-18-3151

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Targeting the Temporal Dynamics of Hypoxia-Induced Tumor-Secreted Factors Halts Tumor Migration
Manjulata Singh, Xiao-Jun Tian, Vera S. Donnenberg, Alan M. Watson, JingYu Zhang, Laura P. Stabile, Simon C. Watkins, Jianhua Xing and Shilpa Sant
Cancer Res June 1 2019 (79) (11) 2962-2977; DOI: 10.1158/0008-5472.CAN-18-3151
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