Skip to main content
  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

AACR logo

  • Register
  • Log in
  • My Cart
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Meeting Abstracts
    • Collections
      • COVID-19 & Cancer Resource Center
      • Focus on Computer Resources
      • Highly Cited Collection
      • Editors' Picks
      • "Best of" Collection
  • For Authors
    • Information for Authors
    • Author Services
    • Early Career Award
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citations
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
  • COVID-19
  • Webinars
  • Search More

    Advanced Search

  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in
  • My Cart

Search

  • Advanced search
Cancer Research
Cancer Research
  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Meeting Abstracts
    • Collections
      • COVID-19 & Cancer Resource Center
      • Focus on Computer Resources
      • Highly Cited Collection
      • Editors' Picks
      • "Best of" Collection
  • For Authors
    • Information for Authors
    • Author Services
    • Early Career Award
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citations
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
  • COVID-19
  • Webinars
  • Search More

    Advanced Search

Poster Session Abstracts

Abstract P6-10-11: In vitro chemo interrogation of viable circulating tumor associated cells from breast cancer patients

Timothy Crook, Dadasaheb Akolkar, Sanket Patil, Vishakha Mhase, Revati Patil, Cynthe Sims, Vineet Datta, Sachin Apurwa, Viwek Mane, Ashok Gawai, Madhavi Apastamb, Ajay Srinivasan and Rajan Datar
Timothy Crook
1Royal Surrey County Hospital, Guildford, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dadasaheb Akolkar
2Datar Cancer Genetics Limited, Nasik, India
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sanket Patil
2Datar Cancer Genetics Limited, Nasik, India
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Vishakha Mhase
2Datar Cancer Genetics Limited, Nasik, India
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Revati Patil
2Datar Cancer Genetics Limited, Nasik, India
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cynthe Sims
2Datar Cancer Genetics Limited, Nasik, India
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Vineet Datta
2Datar Cancer Genetics Limited, Nasik, India
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sachin Apurwa
2Datar Cancer Genetics Limited, Nasik, India
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Viwek Mane
2Datar Cancer Genetics Limited, Nasik, India
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ashok Gawai
2Datar Cancer Genetics Limited, Nasik, India
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Madhavi Apastamb
2Datar Cancer Genetics Limited, Nasik, India
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ajay Srinivasan
2Datar Cancer Genetics Limited, Nasik, India
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rajan Datar
2Datar Cancer Genetics Limited, Nasik, India
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1158/1538-7445.SABCS19-P6-10-11 Published February 2020
  • Article
  • Info & Metrics
Loading
Abstracts: 2019 San Antonio Breast Cancer Symposium; December 10-14, 2019; San Antonio, Texas

Abstract

Background: Chemoresistance in cancer cells is the underlying reason for treatment failure. Real-time monitoring of chemoresistance and response (CRR) is a fundamental but unmet prerequisite of precision oncology. Repetitive invasive biopsies to obtain tumor tissue for in-vitro CRR profiling are neither feasible nor advisable. Circulating Tumor Associated Cells (C-TACs) have been proposed for functional assessment of CRR. In this observational study, we performed CRR profiling on viable C-TACs from breast cancer patients.

Methods: In this 3 arm study, viable C-TACs were harvested from 15 ml of peripheral blood obtained from 242 breast cancer patients using CellWizard™, an epigenetically active media with paradoxical cytotoxicity that selectively kills normal cells and simultaneously confers survival benefit on cells of tumorigenic origin. C-TACs were stained with EPCAM, CK, CD45 and GCDFP. Viable Tumor Derived Cells (TDCs) harvested from freshly biopsied tumor tissue (Arm 1, N=48) and concurrently obtained C-TACs were simultaneously CRR profiled irrespective of whether patient was pre-treated or otherwise. Arm 2 (N=100) included refractory (pre-treated) patients where C-TACs were evaluated for resistance to previously administered drugs. Arm 3 (N=124) included recently diagnosed therapy naïve patients where C-TACs were evaluated for innate chemoresistance.

Results: In Arm 1, CRR profile of C-TACs showed 96.1% concordance with CRR profile of concurrently obtained TDCs. In Arm2, CRR profiling of C-TACs identified chemoresistance due to prior drug exposure in 91.4% of patients. In Arm 3, innate chemoresistance was observed in 42% of patient samples towards commonly used first / second line Standard of Care drugs belonging to various functional categories such as mitotic inhibitors, DNA damaging agents, topo inhibitors and antimetabolites.

Conclusion: This study shows for the first time the feasibility of CRR profiling of C-TACs and its correlation to in-vitro and in-vivo CRR characteristics of the tumor. Adoption of C-TAC based CRR profiling can provide unprecedented real time treatment oversight towards treatment selection and monitoring of drug resistance for personalized treatment of breast cancer patients.

Citation Format: Timothy Crook, Dadasaheb Akolkar, Sanket Patil, Vishakha Mhase, Revati Patil, Cynthe Sims, Vineet Datta, Sachin Apurwa, Viwek Mane, Ashok Gawai, Madhavi Apastamb, Ajay Srinivasan, Rajan Datar. In vitro chemo interrogation of viable circulating tumor associated cells from breast cancer patients [abstract]. In: Proceedings of the 2019 San Antonio Breast Cancer Symposium; 2019 Dec 10-14; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2020;80(4 Suppl):Abstract nr P6-10-11.

  • ©2020 American Association for Cancer Research.
Previous
Back to top
Cancer Research: 80 (4 Supplement)
February 2020
Volume 80, Issue 4 Supplement
  • Table of Contents

Sign up for alerts

Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Abstract P6-10-11: In vitro chemo interrogation of viable circulating tumor associated cells from breast cancer patients
(Your Name) has forwarded a page to you from Cancer Research
(Your Name) thought you would be interested in this article in Cancer Research.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Abstract P6-10-11: In vitro chemo interrogation of viable circulating tumor associated cells from breast cancer patients
Timothy Crook, Dadasaheb Akolkar, Sanket Patil, Vishakha Mhase, Revati Patil, Cynthe Sims, Vineet Datta, Sachin Apurwa, Viwek Mane, Ashok Gawai, Madhavi Apastamb, Ajay Srinivasan and Rajan Datar
Cancer Res February 15 2020 (80) (4 Supplement) P6-10-11; DOI: 10.1158/1538-7445.SABCS19-P6-10-11

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Abstract P6-10-11: In vitro chemo interrogation of viable circulating tumor associated cells from breast cancer patients
Timothy Crook, Dadasaheb Akolkar, Sanket Patil, Vishakha Mhase, Revati Patil, Cynthe Sims, Vineet Datta, Sachin Apurwa, Viwek Mane, Ashok Gawai, Madhavi Apastamb, Ajay Srinivasan and Rajan Datar
Cancer Res February 15 2020 (80) (4 Supplement) P6-10-11; DOI: 10.1158/1538-7445.SABCS19-P6-10-11
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
Advertisement

Related Articles

Cited By...

More in this TOC Section

Poster Session Abstracts

  • Abstract PS18-36: Proteomic profiling of specific tumor clones using spatially resolved mass spectrometry technologies for precision oncology
  • Abstract PS17-24: Inhibition of short-form ron eliminates breast cancer metastases through an immune-mediated mechanism
  • Abstract PS18-07: Association between pathological response and tumor genomic profiling in triple negative breast cancer patients treated with neoadjuvant chemotherapy
Show more Poster Session Abstracts

Predictive Biomarkers - General

  • Abstract P6-10-29: Circular RNAs in exosomes derived from breast cancer: Promising biomarkers for diagnosis and prognosis of triple negative breast cancer (TNBC)
  • Abstract P6-10-30: Developing 3D subtyping model for breast cancer patients based on absolute quantitation or ER, PR and Her2 at protein level
Show more Predictive Biomarkers - General
  • Home
  • Alerts
  • Feedback
  • Privacy Policy
Facebook  Twitter  LinkedIn  YouTube  RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Meeting Abstracts

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians

About Cancer Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2021 by the American Association for Cancer Research.

Cancer Research Online ISSN: 1538-7445
Cancer Research Print ISSN: 0008-5472
Journal of Cancer Research ISSN: 0099-7013
American Journal of Cancer ISSN: 0099-7374

Advertisement