PT  - JOURNAL ARTICLE
AU  - Louis, C. J.
AU  - Blunck, J. M.
TI  - The Isolation of Normal Rat Liver <em>h</em> Proteins and the Immunological Reactions of Mouse Anti-Rat Liver <em>h</em> Protein
DP  - 1970 Jul 01
TA  - Cancer Research
PG  - 2043--2048
VI  - 30
IP  - 7
4099  - http://cancerres.aacrjournals.org/content/30/7/2043.short
4100  - http://cancerres.aacrjournals.org/content/30/7/2043.full
SO  - Cancer Res1970 Jul 01; 30
AB  - Mouse anti-rat liver h protein has been prepared by immunizing mice against h proteins previously isolated from agarose gel slices following preparative electrophoresis of normal rat liver soluble protein. In immunoelectrophoresis and gel double-diffusion procedures, this antibody reacted with normal rat liver h proteins and with soluble liver azoproteins from rats fed azocarcinogen but did not react with soluble proteins from dye-induced rat liver tumors. Autoradiographic and liquid scintillation studies on immunoelectrophoretograms have provided additonal evidence that this antibody is specifically directed against azoproteins. These results are in accord with the deletion of a soluble azoprotein during aminoazo dye carcinogenesis. ©1970 American Association for Cancer Research.