RT Journal Article
SR Electronic
T1 The Isolation of Normal Rat Liver <em>h</em> Proteins and the Immunological Reactions of Mouse Anti-Rat Liver <em>h</em> Protein
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 2043
OP 2048
VO 30
IS 7
A1 Louis, C. J.
A1 Blunck, J. M.
YR 1970
UL http://cancerres.aacrjournals.org/content/30/7/2043.abstract
AB Mouse anti-rat liver h protein has been prepared by immunizing mice against h proteins previously isolated from agarose gel slices following preparative electrophoresis of normal rat liver soluble protein. In immunoelectrophoresis and gel double-diffusion procedures, this antibody reacted with normal rat liver h proteins and with soluble liver azoproteins from rats fed azocarcinogen but did not react with soluble proteins from dye-induced rat liver tumors. Autoradiographic and liquid scintillation studies on immunoelectrophoretograms have provided additonal evidence that this antibody is specifically directed against azoproteins. These results are in accord with the deletion of a soluble azoprotein during aminoazo dye carcinogenesis. ©1970 American Association for Cancer Research.