RT Journal Article
SR Electronic
T1 Phosphorylation of the <em>M</em><sub>r</sub> 170,000 to 180,000 Glycoprotein Specific to Multidrug-resistant Tumor Cells: Effects of Verapamil, Trifluoperazine, and Phorbol Esters
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 2860
OP 2865
VO 47
IS 11
A1 Hamada, Hirofumi
A1 Hagiwara, Ken-Ichi
A1 Nakajima, Terumi
A1 Tsuruo, Takashi
YR 1987
UL http://cancerres.aacrjournals.org/content/47/11/2860.abstract
AB An overexpression of plasma membrane glycoprotein with a relative molecular mass (Mr) of 170,000–180,000 is consistently found in different multidrug-resistant human and animal cell lines, although the functional role of the protein in multidrug resistance is not fully understood. It has been reported previously that the Mr 170,000–180,000 glycoprotein is involved, directly or indirectly, in the drug transport mechanism and the proliferation of multidrug-resistant tumor cells. In an attempt to clarify further the function of the Mr 170,000–180,000 glycoprotein, we have studied the phosphorylation state of the protein in intact K562/ADM cells and found that: (a) the protein is phosphorylated in the basal state; (b) verapamil and trifluoperazine, which inhibit the active drug efflux and restore drug sensitivity in resistant cells, caused an increase in the phosphorylation of the Mr 170,000–180,000 glycoprotein; (c) 4β-phorbol 12β-myristate 13α-acetate and 1-oleoyl 2-acetylglycerol enhanced phosphorylation of the protein; (d) the protein was phosphorylated at serine residues; (e) tryptic phosphopeptide mapping of the Mr 170,000–180,000 glycoprotein showed that 4β-phorbol 12β-myristate 13α-acetate treatment induced an increase in phosphorylation at different sites of the protein from those induced by verapamil or trifluoperazine treatment, suggesting that the protein is phosphorylated by an array of complex regulation mechanisms. Phosphorylation of the Mr 170,000–180,000 glycoprotein might play a role in the regulation of processes affecting cellular function in multidrug resistance. ©1987 American Association for Cancer Research.