RT Journal Article
SR Electronic
T1 Presence of Tumor-associated Antigens in Epidermal Growth Factor Receptors from Different Human Carcinomas
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 2531
OP 2536
VO 47
IS 10
A1 Basu, Aruna
A1 Murthy, Uma
A1 Rodeck, Ulrich
A1 Herlyn, Meenhard
A1 Mattes, Laura
A1 Das, Manjusri
YR 1987
UL http://cancerres.aacrjournals.org/content/47/10/2531.abstract
AB The epidermal growth factor (EGF) receptor in two colon carcinoma lines and in one vulval carcinoma line tested contains carbohydrate determinants that are recognized by monoclonal antibodies to tumor-associated antigens. These antibodies are directed to sialylated Lea and to difucosylated structures of the Y type. Cell lines which react with these antibodies express these antigens on their surface glycolipids and glycoproteins, including the EGF receptor. These unusual carbohydrates are absent in EGF receptors from normal untransformed cells, and from tumor cells which do not express these specific antigens. Although EGF receptor represents only 0.1–2% of total plasma membrane proteins of antigen-positive carcinomas, it accounts for 20–80% of total protein-associated sialylated Lea/Y type of nonsecreted carbohydrates present in these cells. The results of cell-binding, immunoprecipitation, and Western blot analyses of the antigen-positive carcinomas indicate that sialylated Lea/Y type of antigenicity is intrinsic to the EGF receptors of these cells, and that the antigen is present in receptors from both over-expressing and normal-expressing carcinomas. ©1987 American Association for Cancer Research.