RT Journal Article
SR Electronic
T1 Establishment and Characterization of Two New Squamous Cell Carcinoma Cell Lines Derived from Tumors of the Head and Neck
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 2858
OP 2866
VO 48
IS 10
A1 Sacks, Peter G.
A1 Parnes, Steven M.
A1 Gallick, Gary E.
A1 Mansouri, Zahra
A1 Lichtner, Rosemarie
A1 Satya-Prakash, K. L.
A1 Pathak, Sen
A1 Parsons, Donald F.
YR 1988
UL http://cancerres.aacrjournals.org/content/48/10/2858.abstract
AB Two human cell lines were established from untreated squamous cell carcinomas of the head and neck. Line 183 was derived from a head and neck squamous cell carcinoma of the tonsil and 1483 from a head and neck squamous cell carcinoma of the retromolar trigone. Both lines grow in a cobblestone pattern demonstrating their epithelial heritage. Immunofluorescence studies and one-dimensional polyacrylamide gel electrophoresis indicated that both lines contain cytokeratins. Line 1483 is more aggressive in nude mice, has a higher efficiency for anchorage-independent growth, expresses p21ras (product of the ras oncogene) at a higher level, and is more aneuploid than 183. 1483 also grows as a multicellular tumor spheroid. Line 1483, which was established from the primary tumor of a patient with nodal metastasis, thus displays more progressed characteristics than line 183, which was established from a patient with no clinically positive nodes. ©1988 American Association for Cancer Research.