PT  - JOURNAL ARTICLE
AU  - Lijinsky, W.
AU  - Yamashita, K.
TI  - Lack of Binding of Methapyrilene and Similar Antihistamines to Rat Liver DNA Examined by <sup>32</sup>P Postlabeling
DP  - 1988 Nov 15
TA  - Cancer Research
PG  - 6475--6477
VI  - 48
IP  - 22
4099  - http://cancerres.aacrjournals.org/content/48/22/6475.short
4100  - http://cancerres.aacrjournals.org/content/48/22/6475.full
SO  - Cancer Res1988 Nov 15; 48
AB  - The nonmutagenic carcinogen methapyrilene, together with several noncarcinogenic analogues, was administered to rats p.o. for as long as 4 wk at concentrations of 0.1%. DNA was isolated from the liver and other organs and hydrolyzed, and the identification of covalent adducts was made using the 32P postlabeling method of Randerath. Some modified procedures were also used to deal with the possibility of very mobile adducts being formed from these hydrophilic amines. Although the rats had received as much as 2 g of amine per kg of body weight, no evidence of formation of DNA adducts in liver or other organs was seen; the level of detection was between 1 in 108 and 1 in 109 nucleotides. Adduct formation from much lower doses of the mutagenic food pyrolysis product 2-amino-3-methylimidaz(4, 5f)quinoline was detectable at a level of 1 in 106 nucleotides in parallel analyses. These results add to the evidence that carcinogenesis by methapyrilene is through an indirect or nonmutagenic mechanism. ©1988 American Association for Cancer Research.