RT Journal Article
SR Electronic
T1 Susceptibility of Multidrug-resistant Human Leukemia Cell Lines to Human Interleukin 2-activated Killer Cells
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 6793
OP 6799
VO 50
IS 21
A1 Kimmig, Astrid
A1 Gekeler, Volker
A1 Neumann, Manfred
A1 Frese, Gerd
A1 Handgretinger, Rupert
A1 Kardos, Gabriella
A1 Diddens, Heyke
A1 Niethammer, Dietrich
YR 1990
UL http://cancerres.aacrjournals.org/content/50/21/6793.abstract
AB Considering the possibility to overcome drug resistance by other treatment strategies than chemotherapy we investigated the susceptibility of three independently selected multidrug-resistant sublines of the T-lymphoblastoid leukemic cell line CCRF-CEM to lymphokine-activated killer (LAK) cells. We found that two of the multidrug-resistant sublines were significantly less susceptible targets to LAK cells. A third one, however, was as susceptible as the parental CCRF-CEM cell line. Moreover, a multidrug-resistant subline that reverted to an almost drug-sensitive phenotype was observed to be also revertant for resistance against LAK cells. We found an inverse relationship between the expression of the mdr1 gene (P-glycoprotein) and the susceptibility of LAK cells. Verapamil, a calcium channel blocker, while increasing the drug sensitivity of a multidrug-resistant subline, did not induce a reversal of the suppression of LAK susceptibility. The possibility of enhanced resistance to LAK cells of multidrug-resistant cells should be taken into account when one is looking for therapy strategies to overcome multidrug resistance. ©1990 American Association for Cancer Research.