RT Journal Article SR Electronic T1 Expression of the Neuronal Form of pp60c-src in Neuroblastoma in Relation to Clinical Stage and Prognosis JF Cancer Research JO Cancer Res FD American Association for Cancer Research SP 6908 OP 6914 VO 50 IS 21 A1 Bjelfman, Catarina A1 Hedborg, Fredrik A1 Johansson, Irja A1 Nordenskjöld, Magnus A1 Påhlman, Sven YR 1990 UL http://cancerres.aacrjournals.org/content/50/21/6908.abstract AB The expression of the protooncogene c-src has been studied in specimens of childhood tumors with special reference to neuroblastoma and other tumors of neuronal origin. For comparison c-src gene expression was studied in seven neuroblastoma and neuroepithelioma cell lines. The structurally distinct neuronal product of the gene, pp60c-srcN, expressed during normal development in neuroblasts and neurons, was identified by immunoblotting technique together with the fibroblast form, pp60c-src. While pp60c-src was found in most tumors studied, the neuronal form was restricted to neuroblastomas (23 of 27) and retinoblastomas (3 of 3) and could not be detected in the other childhood tumors. A dominance of the neuronal form, pp60c-srcN, was exclusively found in the infant cases of neuroblastoma (9 of 12), estimated to have good prognosis. These results indicate that pp60c-srcN might be a diagnostic marker in primitive childhood tumors. When expressed in higher amounts than pp60c-src, pp60c-srcN may be a positive prognostic marker in neuroblastoma, especially useful in the evaluation of infants. In addition, lack of pp60c-srcN seems to be incompatible with low stage neuroblastoma. ©1990 American Association for Cancer Research.