RT Journal Article SR Electronic T1 A Murine Model for B-Cell Lymphomagenesis in Immunocompromised Hosts: c-myc-rearranged B-Cell Lines with a Premalignant Phenotype JF Cancer Research JO Cancer Res FD American Association for Cancer Research SP 7042 OP 7049 VO 50 IS 21 A1 Felsher, Dean W. A1 Denis, Kathleen A. A1 Weiss, Diane A1 Ando, Dale T. A1 Braun, Jonathan YR 1990 UL http://cancerres.aacrjournals.org/content/50/21/7042.abstract AB Activation of c-myc or bcl-2 protooncogene is a common event in B-cell lymphomagenesis. Alone, each is insufficient to produce lymphoma, prompting the search for the additional steps required to complete the malignant phenotype. Among the existing systems of murine or human B-cell neoplasia, no commonly occurring complementary oncogenic activation has been found. This study introduces a new series of murine B-cell lines with a phenotype suggesting that such additional events might not involve intrinsic growth control, but instead host immune mechanisms which normally suppress tumorigenicity of premalignant B-cells. Four murine B-cell lines were isolated from the long-term culture of normal lymphoid tissue bearing a premalignant phenotype. (a) Their phenotype resembled naturally occurring lymphoid tumors of immunocompromised hosts with regard to c-myc activation, aberrant or absent immunoglobulin expression, preferential rearrangement of the λ light chain locus, and a distinctive pattern of tissue invasion and tumor histology. (b) Their tumorigenicity was strictly dependent on host permissiveness correlated with immunodeficient status: C.B-17-scid > BALB/c-nu/nu > normal BALB/c ≫ other H-2d strains (NZB × NZW F1, NZB, DBA/2). (c) Host passage selected for malignant variants distinguished by a 104-fold increase in tumorigenicity (as judged by limiting cell dose) and by novel tumorigenicity in nonpermissive syngeneic hosts. These features are analogous to properties of human lymphomas arising in immunocompromised states and, to our knowledge, unique among previously reported murine B-cell lines. ©1990 American Association for Cancer Research.