PT  - JOURNAL ARTICLE
AU  - Fleming, Timothy P.
AU  - Saxena, Abha
AU  - Clark, W. Craig
AU  - Robertson, James T.
AU  - Oldfield, Edward H.
AU  - Aaronson, Stuart A.
AU  - Ali, Iqbal Unnisa
TI  - Amplification and/or Overexpression of Platelet-derived Growth Factor Receptors and Epidermal Growth Factor Receptor in Human Glial Tumors
DP  - 1992 Aug 15
TA  - Cancer Research
PG  - 4550--4553
VI  - 52
IP  - 16
4099  - http://cancerres.aacrjournals.org/content/52/16/4550.short
4100  - http://cancerres.aacrjournals.org/content/52/16/4550.full
SO  - Cancer Res1992 Aug 15; 52
AB  - Analysis of genomic organization and expression of platelet-derived growth factor receptors (PDGFR) and epidermal growth factor receptor (EGFR) in human malignant gliomas showed amplification and over-expression of both receptors in distinct subsets of tumors. Amplification of the αPDGFR was detected in 4 of 50 glioblastomas (8%). EGFR was amplified in 9 of the 50 tumors (18%). Western blot analysis showed elevated expression of αPDGFR and EGFR proteins in 4 (24%) and 3 (18%), respectively, of 17 tumor specimens analyzed. Increased production of αPDGFR as well as EGFR proteins was observed in the presence or absence of gene amplification. Three of the 4 tumors with elevated levels of αPDGFR also overexpressed the βPDGFR, which was present as a single copy gene in all 50 tumors analyzed. Our findings suggest that the amplification and/or overexpression either of EGFR or of the αPDGFR along with the coordinate overexpression of the βPDGFR can contribute to the malignant phenotype of distinct subsets of human glioblastoma. ©1992 American Association for Cancer Research.