RT Journal Article
SR Electronic
T1 Amplification and/or Overexpression of Platelet-derived Growth Factor Receptors and Epidermal Growth Factor Receptor in Human Glial Tumors
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 4550
OP 4553
VO 52
IS 16
A1 Fleming, Timothy P.
A1 Saxena, Abha
A1 Clark, W. Craig
A1 Robertson, James T.
A1 Oldfield, Edward H.
A1 Aaronson, Stuart A.
A1 Ali, Iqbal Unnisa
YR 1992
UL http://cancerres.aacrjournals.org/content/52/16/4550.abstract
AB Analysis of genomic organization and expression of platelet-derived growth factor receptors (PDGFR) and epidermal growth factor receptor (EGFR) in human malignant gliomas showed amplification and over-expression of both receptors in distinct subsets of tumors. Amplification of the αPDGFR was detected in 4 of 50 glioblastomas (8%). EGFR was amplified in 9 of the 50 tumors (18%). Western blot analysis showed elevated expression of αPDGFR and EGFR proteins in 4 (24%) and 3 (18%), respectively, of 17 tumor specimens analyzed. Increased production of αPDGFR as well as EGFR proteins was observed in the presence or absence of gene amplification. Three of the 4 tumors with elevated levels of αPDGFR also overexpressed the βPDGFR, which was present as a single copy gene in all 50 tumors analyzed. Our findings suggest that the amplification and/or overexpression either of EGFR or of the αPDGFR along with the coordinate overexpression of the βPDGFR can contribute to the malignant phenotype of distinct subsets of human glioblastoma. ©1992 American Association for Cancer Research.