RT Journal Article SR Electronic T1 Frequent Somatic Mutations of the APC Gene in Human Pancreatic Cancer JF Cancer Research JO Cancer Res FD American Association for Cancer Research SP 6696 OP 6698 VO 52 IS 23 A1 Horii, Akira A1 Nakatsuru, Shuichi A1 Miyoshi, Yasuo A1 Ichii, Shigetoshi A1 Nagase, Hiroki A1 Ando, Hiroshi A1 Yanagisawa, Akio A1 Tsuchiya, Eiju A1 Kato, Yo A1 Nakamura, Yusuke YR 1992 UL http://cancerres.aacrjournals.org/content/52/23/6696.abstract AB The APC (adenomatous polyposis coli) gene is responsible for familial adenomatous polyposis and is also associated with the development of sporadic tumors of the colon and stomach. To investigate whether or not mutations of APC play any role in tumors arising in other organs, we examined somatic mutations of this gene in sporadic (nonfamilial) renal cell carcinomas, hepatocellular carcinomas, and cancers of the lung and pancreas. DNAs isolated from tumors were examined by means of a RNase protection analysis, coupled with the polymerase chain reaction followed by DNA sequencing of the polymerase chain reaction products. By screening a part of the APC coding region, we detected somatic mutations in four of ten pancreatic cancers; each of these mutations would yield a truncated APC product due to a 1- or S-base pair deletion. These results imply that mutations in APC contribute to carcinogenesis in the pancreas. ©1992 American Association for Cancer Research.