PT  - JOURNAL ARTICLE
AU  - Economos, Katherine
AU  - MacDonald, Paul C.
AU  - Casey, M. Linette
TI  - Endothelin-1 Gene Expression and Biosynthesis in Human Endometrial HEC-1A Cancer Cells
DP  - 1992 Feb 01
TA  - Cancer Research
PG  - 554--557
VI  - 52
IP  - 3
4099  - http://cancerres.aacrjournals.org/content/52/3/554.short
4100  - http://cancerres.aacrjournals.org/content/52/3/554.full
SO  - Cancer Res1992 Feb 01; 52
AB  - In this study, evidence was obtained that endothelin-1 (ET-1) is produced by an established endometrial cancer (HEC-1A) cell line. PreproET-1 mRNA is present in HEC-1A cells, and immunoreactive endothelin is secreted into the medium of these cells maintained in culture. Cycloheximide treatment of these cells caused superinduction of preproET-1 mRNA. Transforming growth factor-β acts in these cells to increase the levels of preproET-1 mRNA. This effect of transforming growth factor-β on preproET-1 mRNA accumulation was accompanied by an increase in the amount of immunoreactive endothelin secreted into the culture medium. ET-1, added to the culture medium, did not act as a mitogen in HEC-1A cells. We speculate that ET-1 (which is known to stimulate fibroblast proliferation) produced by endometrial adenocarcinoma cells may participate in the angiogenic process that occurs during the establishment of this carcinoma in vivo. ©1992 American Association for Cancer Research.