RT Journal Article
SR Electronic
T1 Levels of Matrix Metalloproteases in Bladder Cancer Correlate with Tumor Grade and Invasion
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 5365
OP 5369
VO 53
IS 22
A1 Davies, Bernard
A1 Waxman, Jonathan
A1 Wasan, Harpret
A1 Abel, Paul
A1 Williams, Gordon
A1 Krausz, Thomas
A1 Neal, David
A1 Thomas, David
A1 Hanby, Andrew
A1 Balkwill, Frances
YR 1993
UL http://cancerres.aacrjournals.org/content/53/22/5365.abstract
AB We have used quantitative zymography to measure levels of the type IV collagenases matrix metalloproteinase (MMP)-9 and MMP-2 in 42 biopsies of transitional cell carcinoma and in 7 biopsies of normal bladder. Mean levels of MMP-9 were significantly higher in tumor compared with normal samples (P = 0.08). Levels of MMP-9 and active MMP-2 increased with tumor grade (test for trend, P = 0.002 and P = 0.05, respectively). Levels of MMP-9 and activated MMP-2 were also higher in invasive tumors than in superficial ones (P = 0.001 and P = 0.008, respectively). In situ hybridization studies showed that the mRNAs for both MMP-2 and MMP-9 were located chiefly in the stroma rather than epithelial tumor cells and were concentrated at the interface between the two tissues. ©1993 American Association for Cancer Research.