RT Journal Article
SR Electronic
T1 Inhibition of Growth of Prostatic Cancer Cell Lines by Peptide Analogues of Insulin-like Growth Factor 1
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 1102
OP 1106
VO 53
IS 5
A1 Pietrzkowski, Zbigniew
A1 Mulholland, Grant
A1 Gomella, Leonard
A1 Jameson, Bradford A.
A1 Wernicke, Dorothee
A1 Baserga, Renato
YR 1993
UL http://cancerres.aacrjournals.org/content/53/5/1102.abstract
AB We have investigated three prostatic cancer cell lines, PC-3, DU-145, and LNCa.FGC, and found that all three cell lines can grow in serum-free medium without the addition of exogenous growth factors. All three cell lines produce substantial amounts of insulin-like growth factor 1 (IGF-1) that is secreted in the medium and they all display constitutively autophosphorylated IGF-1 receptors; two of the cell lines overexpress IGF-1 receptor RNA. The growth of all three cell lines is inhibited by an antisense oligodeoxynucleotide to IGF-1 receptor RNA or by peptide analogues of IGF-1 that compete with IGF-1 for binding to its receptor. Our results indicate that these three cell lines grow by an autocrine loop in which the overproduced IGF-1 activates its receptor. Interference with the activation of the receptor leads to cessation of growth. ©1993 American Association for Cancer Research.