RT Journal Article
SR Electronic
T1 Isolation of Differentially Expressed Sequence Tags from Human Breast Cancer
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 4598
OP 4602
VO 54
IS 17
A1 Watson, Mark A.
A1 Fleming, Timothy P.
YR 1994
UL http://cancerres.aacrjournals.org/content/54/17/4598.abstract
AB Identification of quantitative changes in gene expression that occur in the malignant mammary gland, if sufficiently characterized, may yield novel molecular markers which may be useful in the diagnosis and treatment of human breast cancer. Using modifications of a previously documented technique, the differential display polymerase chain reaction, we describe the isolation of differentially expressed sequence tags, short complementary DNA fragments corresponding to mRNAs that are differentially expressed in breast cancer biopsies, as compared to normal breast tissue controls. Direct sequencing and expression analysis of two sequence tags demonstrate that they represent sequences which are overexpressed in a number of breast carcinoma cell lines. A paradigm for generating a catalogue of these sequence tags is discussed. ©1994 American Association for Cancer Research.