PT  - JOURNAL ARTICLE
AU  - Wieland, Ilse
AU  - Böhm, Malte
TI  - Frequent Allelic Deletion at a Novel Locus on Chromosome 5 in Human Lung Cancer
DP  - 1994 Apr 01
TA  - Cancer Research
PG  - 1772--1774
VI  - 54
IP  - 7
4099  - http://cancerres.aacrjournals.org/content/54/7/1772.short
4100  - http://cancerres.aacrjournals.org/content/54/7/1772.full
SO  - Cancer Res1994 Apr 01; 54
AB  - Frequent allelic deletions in tumor cells are indicative of the inactivation of tumor suppressor genes. Recently, we isolated the single-copy sequence del-27 (I. Wieland, M. Böhm, and S. Bogatz, Proc. Natl. Acad. Sci. USA, 89: 9705–9709, 1992). Here we show that del-27 detects a restriction fragment length polymorphism that allows examination for loss of heterozygosity (LOH) in tumor specimens. LOH at the del-27 locus occurred in 57% (4 of 7) of the informative lung carcinomas independent of the histopathological differentiation grade. LOH for exon 11 of the APC gene occurred in 71% (5 of 7) of the informative cases but was not associated with LOH at the del-27 locus. The del-27 sequence was localized to chromosome 5p13–5q14, proximal to the MCC/APC region, using a somatic cell hybrid panel. Together with our previous finding that del-27 is deleted homozygously in a lung carcinoma cell line, these results suggest that del-27 is linked closely to a novel putative tumor suppressor gene. ©1994 American Association for Cancer Research.