RT Journal Article
SR Electronic
T1 Codeletion of <em>p15</em> and <em>p16</em> Genes in Primary Non-Small Cell Lung Carcinoma
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 2968
OP 2971
VO 55
IS 14
A1 Xiao, Sheng
A1 Li, Daizong
A1 Corson, Joseph M.
A1 Vijg, Jan
A1 Fletcher, Jonathan A.
YR 1995
UL http://cancerres.aacrjournals.org/content/55/14/2968.abstract
AB Chromosome band 9p21 is deleted frequently in non-small cell lung carcinoma (NSCLC), and the p15 and p16 cyclin-dependent kinase-4 inhibitor genes map within this deletion region. Recent studies demonstrated deletion of p15 and p16 in NSCLC metastases and cell lines, suggesting a role for these genes in NSCLC progression. We now report p15 and p16 copy number, as determined by fluorescence in situ hybridization with a P1 contig, in 18 primary NSCLCs. Codeletion of p15 and p16 was found in 15 of 18 NSCLCs, and 1 of the 3 tumors with normal p15 and p16 copy number had a nonsense mutation in exon 2 of p16. We conclude that p15 and p16 are deleted and/or mutated in most primary NSCLCs. Two observations, however, support the involvement of at least one additional tumor suppressor gene on chromosome 9. These observations are: (a) the large size (&gt; 100 kb) of most NSCLC p15/p16 deletions; and (b) the absence of exon 2 mutations in most retained NSCLC p15 and p16 alleles. ©1995 American Association for Cancer Research.