RT Journal Article
SR Electronic
T1 Alteration of Glyceraldehyde-3-phosphate Dehydrogenase Activity and Messenger RNA Content by Androgen in Human Prostate Carcinoma Cells
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 4234
OP 4236
VO 55
IS 19
A1 Ripple, Maureen O.
A1 Wilding, George
YR 1995
UL http://cancerres.aacrjournals.org/content/55/19/4234.abstract
AB Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) activity and mRNA content is altered in the androgen-responsive human prostate carcinoma cell line LNCaP after exposure to the synthetic androgen R1881. Elevation in GAPDH activity is noted as early as 24 h after treatment with 1 nm R1881 and lasts at least 96 h. R1881 has no effect on the activity of GAPDH in androgen-independent DU145 cells. LNCaP GAPDH mRNA content is lowered by treatment with 1 nm R1881; the magnitude of reduction appears to depend on the length of exposure. The results present at least one means by which androgen-responsive tissues may develop alterations in GAPDH mRNA or activity, as is found in certain tumor tissues. ©1995 American Association for Cancer Research.