RT Journal Article
SR Electronic
T1 Suppression of Saccharin-induced Mutagenicity by Interferon-α in Human RSa Cells
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 4253
OP 4256
VO 55
IS 19
A1 Suzuki, Nobuo
A1 Suzuki, Hideko
YR 1995
UL http://cancerres.aacrjournals.org/content/55/19/4253.abstract
AB Saccharin is an artificial sweetener commonly used in the formulation of foods and beverages. Sodium saccharin-induced mutagenicity is detectable in human RSa cells by estimation of cloning efficiency of ouabainresistant mutant cells and determination of K-ras codon 12 mutation in genomic DNA, analyzed by PCR and differential dot-blot hybridization. However, in this study no phenotypic or genetic mutations were detected in RSa cells cultured with human IFN (HuIFN)-α before sodium saccharin treatment. The suppressive effect was lessened by transient treatment with antipain immediately after sodium saccharin treatment. Elevation of antipain-sensitive protease activity was found, furthermore, in RSa cells cultured with HuIFN-α and subsequently treated with sodium saccharin. Thus, antipain-sensitive protease induction in cells tested here may be involved in suppression of the mutagenicity of saccharin by HuIFN-α. ©1995 American Association for Cancer Research.