PT  - JOURNAL ARTICLE
AU  - Felix, Carolyn A.
AU  - Lange, Beverly J.
AU  - Hosler, Matthew R.
AU  - Fertala, Jolanta
AU  - Bjornsti, Mary-Ann
TI  - Chromosome Band 11q23 Translocation Breakpoints Are DNA Topoisomerase II Cleavage Sites
DP  - 1995 Oct 01
TA  - Cancer Research
PG  - 4287--4292
VI  - 55
IP  - 19
4099  - http://cancerres.aacrjournals.org/content/55/19/4287.short
4100  - http://cancerres.aacrjournals.org/content/55/19/4287.full
SO  - Cancer Res1995 Oct 01; 55
AB  - Human leukemias with 11q23 translocations occur sporadically and after cancer treatment with DNA topoisomerase II-targeted drugs. To investigate this process, we examined DNA topoisomerase II cleavage in vitro in subclones of the normal 11q23 genomic homologue and a t(9;11) translocation breakpoint junction. Cleavage was assayed with limiting dilutions of enzyme in the presence or absence of epipodophyllotoxin and ATP. The strongest sites of cleavage coincided with the t(9;11) breakpoint site and two other translocation breakpoint sites within the normal homologue. These results support the involvement of DNA topoisomerase II in the translocation process at chromosome band 11q23. ©1995 American Association for Cancer Research.