RT Journal Article
SR Electronic
T1 Chromosome Band 11q23 Translocation Breakpoints Are DNA Topoisomerase II Cleavage Sites
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 4287
OP 4292
VO 55
IS 19
A1 Felix, Carolyn A.
A1 Lange, Beverly J.
A1 Hosler, Matthew R.
A1 Fertala, Jolanta
A1 Bjornsti, Mary-Ann
YR 1995
UL http://cancerres.aacrjournals.org/content/55/19/4287.abstract
AB Human leukemias with 11q23 translocations occur sporadically and after cancer treatment with DNA topoisomerase II-targeted drugs. To investigate this process, we examined DNA topoisomerase II cleavage in vitro in subclones of the normal 11q23 genomic homologue and a t(9;11) translocation breakpoint junction. Cleavage was assayed with limiting dilutions of enzyme in the presence or absence of epipodophyllotoxin and ATP. The strongest sites of cleavage coincided with the t(9;11) breakpoint site and two other translocation breakpoint sites within the normal homologue. These results support the involvement of DNA topoisomerase II in the translocation process at chromosome band 11q23. ©1995 American Association for Cancer Research.