PT  - JOURNAL ARTICLE
AU  - Yokomizo, Akira
AU  - Ono, Mayumi
AU  - Nanri, Hiroki
AU  - Makino, Yoshinari
AU  - Ohga, Takefumi
AU  - Wada, Morimasa
AU  - Okamoto, Takashi
AU  - Yodoi, Junji
AU  - Kuwano, Michihiko
AU  - Kohno, Kimitoshi
TI  - Cellular Levels of Thioredoxin Associated with Drug Sensitivity to Cisplatin, Mitomycin C, Doxorubicin, and Etoposide
DP  - 1995 Oct 01
TA  - Cancer Research
PG  - 4293--4296
VI  - 55
IP  - 19
4099  - http://cancerres.aacrjournals.org/content/55/19/4293.short
4100  - http://cancerres.aacrjournals.org/content/55/19/4293.full
SO  - Cancer Res1995 Oct 01; 55
AB  - Thioredoxin, a cellular thiol, functions as a self-defense mechanism in response to environmental stimuli, including oxidative stress. We first determined cellular levels of thioredoxin in several human bladder and prostatic cancer cell lines resistant to cis-diamminedichloroplatinum(II) (cisplatin). All cisplatin-resistant cell lines had much higher levels of thioredoxin than those in their drug-sensitive parental counterpart. We then, by introducing thioredoxin antisense expression plasmids into human bladder cancer T24 cells, established two bladder cancer cell lines that had decreased levels of thioredoxin. These thioredoxin antisense transfectants showed increased sensitivity to cisplatin and also to other superoxide-generating agents, i.e., doxorubicin, mitomycin C, etoposide, and hydrogen peroxide, as well as to UV irradiation, but not to the tubulin-targeting agents, vincristine, and colchicine. Cellular levels of thioredoxin thus appear to limit sensitivity to various superoxide-generating anticancer drugs in cancer cells. ©1995 American Association for Cancer Research.