RT Journal Article
SR Electronic
T1 Migration of Coordinated Cell Clusters in Mesenchymal and Epithelial Cancer Explants <em>in Vitro</em>
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 4557
OP 4560
VO 55
IS 20
A1 Friedl, Peter
A1 Noble, Peter B.
A1 Walton, Paul A.
A1 Laird, Dale W.
A1 Chauvin, Peter J.
A1 Tabah, Roger J.
A1 Black, Martin
A1 Zänker, Kurt S.
YR 1995
UL http://cancerres.aacrjournals.org/content/55/20/4557.abstract
AB The invasion and migration occurring in primary neoplastic tissue explants were studied by using a three-dimensional collagen matrix model, subsequent time-lapse videomicroscopy, and computer-assisted cell tracking. We show that not only single cells but groups of clustered cells comprising 5 to more than 100 cells detach from the primary tumor lesion and migrate within the adjacent extracellular matrix. These clusters were highly polarized, resulting in a high directional persistence of migration. Locomoting cell clusters were observed in primary cultures from invasive oral squamous cell carcinomas (6 of 9), ductal breast carcinomas (2 of 3), and rhabdomyosarcoma (1 of 1), whereas normal oral mucosa (0 of 4) was cell cluster negative. Thus, locomoting cell clusters could be a novel and potentially important mechanism of cancer cell invasion and metastasis. ©1995 American Association for Cancer Research.