RT Journal Article
SR Electronic
T1 Apoptosis, Cell Replication, and Western-style Diet-induced Tumorigenesis in Mouse Colon
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 4910
OP 4916
VO 56
IS 21
A1 Risio, Mauro
A1 Lipkin, Martin
A1 Newmark, Harold
A1 Yang, Kan
A1 Rossini, Francesco P.
A1 Steele, Vernon E.
A1 Boone, Charles W.
A1 Kelloff, Gary J.
YR 1996
UL http://cancerres.aacrjournals.org/content/56/21/4910.abstract
AB In this study, feeding Western-style diets (WDs) to mice for a duration of two years without any chemical carcinogen led to the development of gross colonic lesions that were histologically classified as dysplastic crypts and focal hyperplasias with or without atypical nuclei. To better understand early biological events contributing to the development of colonic neoplasia, grossly normal colonic mucosa was investigated; mitotic and apoptotic colonic epithelial cells, atypical mitosis, and atypical nuclei were studied. A significant and transient increase of mitotic activity in the basal and intermediate portions of the colonic crypts was seen in young mice after feeding them the WDs. This was accompanied by diffuse activation of apoptosis of the colonic epithelial cells. In the middle of the rodents' life span, after administration of both the WDs and control diet, the rodents developed a marked depletion of apoptotic epithelial cells in the mid-region of the colonic crypts; this was followed by the expansion of an epithelial cell population containing atypical nuclei, and the emergence of the gross lesions noted above. With this sequence of events, prolonged feeding of WDs to mice produced single-crypt dysplastic lesions and focal hyperplasias indicative of tumorigenesis. ©1996 American Association for Cancer Research.