PT  - JOURNAL ARTICLE
AU  - Gomez-Manzano, Candelaria
AU  - Fueyo, Juan
AU  - Kyritsis, Athanassios P.
AU  - Steck, Peter A.
AU  - Roth, Jack A.
AU  - McDonnell, Timothy J.
AU  - Steck, Kim D.
AU  - Levin, Victor A.
AU  - Alfred Yung, W. K.
TI  - Adenovirus-mediated Transfer of the <em>p53</em> Gene Produces Rapid and Generalized Death of Human Glioma Cells via Apoptosis
DP  - 1996 Feb 15
TA  - Cancer Research
PG  - 694--699
VI  - 56
IP  - 4
4099  - http://cancerres.aacrjournals.org/content/56/4/694.short
4100  - http://cancerres.aacrjournals.org/content/56/4/694.full
SO  - Cancer Res1996 Feb 15; 56
AB  - Wild-type p53 is involved in several aspects of cell cycle control and suppression of transformation, inducing either apoptosis or G1 block in cell cycle progression. Using a recombinant adenovirus containing the wild-type p53 cDNA, the biological effects of the newly expressed wild-type p53 protein were examined in six human glioma cell lines. Three cell lines (U-251 MG, U-373 MG, and A-172) expressed endogenous mutant p53, and the other three (U-87 MG, EFC-2, and D54 MG) expressed wild-type p53. The restoration of normal p53-encoded protein in the mutant cell lines induced apoptosis as assessed by morphological studies using nuclear staining, electron microscopy, and flow cytometric assays. In wild-type p53 cell lines, however, the overexpression of wild-type p53 did not result in apoptosis but inhibited cellular proliferation rather drastically and modified the neoplastic phenotype. Differential effects suggest two pathways for glioma oncogenesis and a possible therapeutic strategy. ©1996 American Association for Cancer Research.