RT Journal Article
SR Electronic
T1 Somatic Mutations of <em>PTEN</em> in Glioblastoma Multiforme
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 4183
OP 4186
VO 57
IS 19
A1 Wang, Steven I.
A1 Puc, Janusz
A1 Li, Jing
A1 Bruce, Jeffrey N.
A1 Cairns, Paul
A1 Sidransky, David
A1 Parsons, Ramon
YR 1997
UL http://cancerres.aacrjournals.org/content/57/19/4183.abstract
AB Alterations of the PTEN gene occur in glioblastoma multiforme. To determine the frequency of PTEN alteration, 34 consecutive glioblastomas were studied in detail. Sequencing each of the nine exons amplified from tumor DNA revealed 11 mutations. Analysis of polymorphic markers within and surrounding the PTEN gene identified an additional four homozygous deletion mutations. Loss of heterozygosity (LOH) was observed in 25 of 34 (74%) cases. All mutations occurred in the presence of LOH. PTEN was mutated in 44% (15 of 34) of all glioblastomas studied and 60% (15 of 25) of tumors with LOH on 10q. Thus, PTEN appears to be the major target of inactivation on chromosome 10q in glioblastoma multiforme. ©1997 American Association for Cancer Research.