RT Journal Article
SR Electronic
T1 eps15 and eps15R Are Essential Components of the Endocytic Pathway
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 5498
OP 5504
VO 57
IS 24
A1 Carbone, Roberta
A1 Fré, Silvia
A1 Iannolo, Gioacchin
A1 Belleudi, Francesca
A1 Mancini, Patrizia
A1 Pelicci, Pier Giuseppe
A1 Torrisi, Maria Rosaria
A1 Di Fiore, Pier Paolo
YR 1997
UL http://cancerres.aacrjournals.org/content/57/24/5498.abstract
AB eps15 and eps15R are substrates of the epidermal growth factor (EGF) receptor kinase that are characterized by the presence of a protein:protein interaction domain, the EH domain, and by their ability to bind to the clathrin adaptor protein complex adaptor protein 2. Indirect evidence suggests that eps15 and eps15R are involved in endocytosis. Here we show that microinjection of antibodies against eps15 and eps15R inhibits internalization of EGF and transferrin. In addition, fragments of eps15 (encompassing its EH domains or the COOH-terminal region that binds to adaptor protein 2) inhibit EGF internalization or endocytosis of Sindbis virus. These results demonstrate that eps15 and eps15R are essential components of the endocytic machinery. ©1997 American Association for Cancer Research.