RT Journal Article
SR Electronic
T1 The Helix-Loop-Helix Protein Id2 Is Overexpressed in Human Pancreatic Cancer
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 3769
OP 3772
VO 58
IS 17
A1 Kleeff, Jörg
A1 Ishiwata, Toshiyuki
A1 Friess, Helmut
A1 Büchler, Markus W.
A1 Israel, Mark A.
A1 Korc, Murray
YR 1998
UL http://cancerres.aacrjournals.org/content/58/17/3769.abstract
AB Id2 belongs to the Id family of helix-loop-helix (HLH) proteins, which upon heterodimerization with basic HLH proteins prevent basic HLH proteins from DNA binding. Proteins of the Id family act as negative regulatory transcriptional factors, and their expression correlates with cell proliferation and arrested differentiation in many cell lineages. In this study, we characterized the expression of Id2 in normal and cancerous pancreatic tissues. Pancreatic cancers markedly overexpressed Id2 mRNA in comparison to the normal pancreas. Furthermore, there was abundant Id2 immunoreactivity in the cancer cells within the pancreatic tumor mass. In PANC-1 human pancreatic cancer cells, steady-state Id2 mRNA levels increased upon serum addition and decreased after induction of differentiation with either sodium butyrate or 12-O-tetradecanoylphorbol-13-acetate. Inhibition of Id2 expression with Id2 antisense oligonucleotides inhibited the growth of these cells, whereas random and sense oligonucleotides were without effect. These findings suggest that Id2 may have a role in human pancreatic cancer. ©1998 American Association for Cancer Research.