PT  - JOURNAL ARTICLE
AU  - Esteller, Manel
AU  - Corn, Paul G.
AU  - Urena, Jesus M.
AU  - Gabrielson, Edward
AU  - Baylin, Stephen B.
AU  - Herman, James G.
TI  - Inactivation of Glutathione <em>S</em>-Transferase <em>P1</em> Gene by Promoter Hypermethylation in Human Neoplasia
DP  - 1998 Oct 15
TA  - Cancer Research
PG  - 4515--4518
VI  - 58
IP  - 20
4099  - http://cancerres.aacrjournals.org/content/58/20/4515.short
4100  - http://cancerres.aacrjournals.org/content/58/20/4515.full
SO  - Cancer Res1998 Oct 15; 58
AB  - Glutathione S-transferases (GSTs) are a family of isoenzymes that play an important role in protecting cells from cytotoxic and carcinogenic agents. The π-class GST has been associated with preneoplastic and neoplastic changes. Recently, it has been reported that regulatory sequences near the GSTP1 gene, which encodes the human π-class GST, are commonly hypermethylated in prostatic carcinomas. In the present study, we studied more than 300 primary human tumors originating in other organs for aberrant methylation of GSTP1 using methylation-specific PCR. GSTP1 hypermethylation was most frequent in breast and renal carcinoma, showing aberrant methylation in 30 and 20% of the cases, respectively. Other tumor types showed promoter methylation only rarely or not at all. Hypermethylation of GSTP1 was associated with loss of expression demonstrated by immunohistochemistry. Our results suggest that aberrant methylation of GSTP1 may contribute to the carcinogenetic process in breast and renal carcinomas. ©1998 American Association for Cancer Research.