PT  - JOURNAL ARTICLE
AU  - Simon, Babette
AU  - Bartsch, Detlef
AU  - Barth, Peter
AU  - Prasnikar, Nicole
AU  - Münch, Karin
AU  - Blum, Andreas
AU  - Arnold, Rudolf
AU  - Göke, Burkhard
TI  - Frequent Abnormalities of the Putative Tumor Suppressor Gene <em>FHIT</em> at 3p14.2 in Pancreatic Carcinoma Cell Lines
DP  - 1998 Apr 15
TA  - Cancer Research
PG  - 1583--1587
VI  - 58
IP  - 8
4099  - http://cancerres.aacrjournals.org/content/58/8/1583.short
4100  - http://cancerres.aacrjournals.org/content/58/8/1583.full
SO  - Cancer Res1998 Apr 15; 58
AB  - The FHIT gene is localized on chromosome 3p14, a region including a tumor cell-specific, commonly deleted region. To determine the role of the FHIT gene in pancreatic carcinogenesis, 14 pancreatic carcinoma cell lines were analyzed by reverse transcription-PCR and exon-specific PCR amplification of genomic DNA. The full-length FHIT transcript was lost in 70% of the pancreatic carcinoma cell lines analyzed, while 66% also revealed intragenic homozygous deletions of exons, 3, 4, and 5. Truncated FHIT transcripts lacking a variable number of exons most likely represented alternative splicing products. Fhit protein expression was dependent on a full-length FHIT transcript. The results suggest that the FHIT gene may be a target tumor suppressor gene involved in pancreatic carcinogenesis. ©1998 American Association for Cancer Research.