RT Journal Article
SR Electronic
T1 Frequent Abnormalities of the Putative Tumor Suppressor Gene <em>FHIT</em> at 3p14.2 in Pancreatic Carcinoma Cell Lines
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 1583
OP 1587
VO 58
IS 8
A1 Simon, Babette
A1 Bartsch, Detlef
A1 Barth, Peter
A1 Prasnikar, Nicole
A1 Münch, Karin
A1 Blum, Andreas
A1 Arnold, Rudolf
A1 Göke, Burkhard
YR 1998
UL http://cancerres.aacrjournals.org/content/58/8/1583.abstract
AB The FHIT gene is localized on chromosome 3p14, a region including a tumor cell-specific, commonly deleted region. To determine the role of the FHIT gene in pancreatic carcinogenesis, 14 pancreatic carcinoma cell lines were analyzed by reverse transcription-PCR and exon-specific PCR amplification of genomic DNA. The full-length FHIT transcript was lost in 70% of the pancreatic carcinoma cell lines analyzed, while 66% also revealed intragenic homozygous deletions of exons, 3, 4, and 5. Truncated FHIT transcripts lacking a variable number of exons most likely represented alternative splicing products. Fhit protein expression was dependent on a full-length FHIT transcript. The results suggest that the FHIT gene may be a target tumor suppressor gene involved in pancreatic carcinogenesis. ©1998 American Association for Cancer Research.